Comprehensive ex vivo and in vivo preclinical evaluation of novel chemo enzymatic decellularized peripheral nerve allografts

被引:5
作者
Garcia-Garcia, Oscar Dario [1 ,2 ,3 ,4 ,5 ]
El Soury, Marwa [1 ,2 ,4 ,5 ]
Campos, Fernando [1 ,2 ]
Sanchez-Porras, David [1 ,2 ]
Geuna, Stefano [4 ,5 ]
Alaminos, Miguel [1 ,2 ]
Gambarotta, Giovanna [4 ,5 ]
Chato-Astrain, Jesus [1 ,2 ]
Raimondo, Stefania [4 ,5 ]
Carriel, Victor [1 ,2 ]
机构
[1] Univ Granada, Dept Histol, Tissue Engn Grp, Granada, Spain
[2] Inst Invest Biosanitaria ibs GRANADA, Granada, Spain
[3] Univ Granada, Doctoral Program Biomed, Granada, Spain
[4] Univ Torino, Dept Clin & Biol Sci, Orbassano, Italy
[5] Univ Torino, Neurosci Inst Cavalieri Ottolenghi NICO, Orbassano, Italy
关键词
acellular graft; decellularization; decellularized nerve allograft; peripheral nerve repair; tissue engineering; MESENCHYMAL STEM-CELLS; RAT SCIATIC-NERVE; NEURAL TISSUE; BONE-MARROW; REGENERATION; CONDUITS; REPAIR; INJURY; SCAFFOLDS; DEFECTS;
D O I
10.3389/fbioe.2023.1162684
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
As a reliable alternative to autografts, decellularized peripheral nerve allografts (DPNAs) should mimic the complex microstructure of native nerves and be immunogenically compatible. Nevertheless, there is a current lack of decellularization methods able to remove peripheral nerve cells without significantly altering the nerve extracellular matrix (ECM). The aims of this study are firstly to characterize ex vivo, in a histological, biochemical, biomechanical and ultrastructural way, three novel chemical-enzymatic decellularization protocols (P1, P2 and P3) in rat sciatic nerves and compared with the Sondell classic decellularization method and then, to select the most promising DPNAs to be tested in vivo. All the DPNAs generated present an efficient removal of the cellular material and myelin, while preserving the laminin and collagen network of the ECM (except P3) and were free from any significant alterations in the biomechanical parameters and biocompatibility properties. Then, P1 and P2 were selected to evaluate their regenerative effectivity and were compared with Sondell and autograft techniques in an in vivo model of sciatic defect with a 10-mm gap, after 15 weeks of follow-up. All study groups showed a partial motor and sensory recovery that were in correlation with the histological, histomorphometrical and ultrastructural analyses of nerve regeneration, being P2 the protocol showing the most similar results to the autograft control group.
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页数:22
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