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A novel chalcone derivative exerts anticancer effects by promoting apoptotic cell death of human pancreatic cancer cells
被引:2
作者:

Baek, Suji
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UMUST R&D Corp, Res & Dev Ctr, Seoul 01411, South Korea UMUST R&D Corp, Res & Dev Ctr, Seoul 01411, South Korea

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Park, Joo Yeon
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UMUST R&D Corp, Res & Dev Ctr, Seoul 01411, South Korea UMUST R&D Corp, Res & Dev Ctr, Seoul 01411, South Korea

Lee, Sang Ju
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Univ Ulsan, Coll Med, Asan Med Ctr, Dept Nucl Med, Seoul 05505, South Korea UMUST R&D Corp, Res & Dev Ctr, Seoul 01411, South Korea

Kang, Yong Gil
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UMUST R&D Corp, Res & Dev Ctr, Seoul 01411, South Korea UMUST R&D Corp, Res & Dev Ctr, Seoul 01411, South Korea

Kwon, Seung Hae
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Korea Basic Sci Inst, Seoul Ctr, Seoul 02841, South Korea UMUST R&D Corp, Res & Dev Ctr, Seoul 01411, South Korea

Oh, Seung Jun
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Univ Ulsan, Coll Med, Asan Med Ctr, Dept Nucl Med, Seoul 05505, South Korea UMUST R&D Corp, Res & Dev Ctr, Seoul 01411, South Korea

Lee, Kang Pa
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UMUST R&D Corp, Res & Dev Ctr, Seoul 01411, South Korea UMUST R&D Corp, Res & Dev Ctr, Seoul 01411, South Korea

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机构:
[1] UMUST R&D Corp, Res & Dev Ctr, Seoul 01411, South Korea
[2] Korea Basic Sci Inst, Seoul Ctr, Seoul 02841, South Korea
[3] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Nucl Med, Seoul 05505, South Korea
[4] Ewha Womans Univ, Seoul Hosp, Dept Nucl Med, Coll Med, Seoul 07804, South Korea
基金:
新加坡国家研究基金会;
关键词:
Chalcone;
Pancreatic cancer;
Apoptosis;
Lipid accumulation;
Stimulated Raman scattering microscopy;
D O I:
10.1016/j.bmc.2023.117458
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Aggressive pancreatic cancer is typically treated using chemotherapeutics to reduce the tumor pre-operatively and prevent metastasis post-operatively, as well as surgical approaches. In the present study, we synthesized a hydroxyl group-introduced chalcone derivative (1, IC50 = 32.1 & mu;M) and investigated its potential as an anticancer drug candidate by evaluating its apoptosis-promoting effects on BXPC-3 cancer cells. The viability of BXPC-3 cells treated with 1 was measured using the water-soluble tetrazolium 1 reagent. BXPC-3 cells induced by 1 were stained with diverse probes or antibodies, such as ethidium homodimer-1, Hoechst, anti-Ki67, and MitoTracker. Protein expression was measured using an immunoblotting assay, and mRNA expression was determined using real-time polymerase chain reaction. Apoptotic molecular features, such as lipid accumulation and protein degradation, were monitored directly using stimulated Raman scattering microspectroscopy. Through incubation time-and concentration-dependent studies of 1, we found that it significantly reduced the proliferation and increased the number of apoptotic BXPC-3 cells. Compound 1 induced mitochondrial dysfunction, phosphorylation of p38, and caspase 3 cleavage. These results indicate that 1 is a potential therapeutic agent for pancreatic cancer, providing valuable insights into the development of new anticancer drug candidates.
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[1]
Single-droplet surface-enhanced Raman scattering decodes the molecular determinants of liquid-liquid phase separation
[J].
Avni, Anamika
;
Joshi, Ashish
;
Walimbe, Anuja
;
Pattanashetty, Swastik G.
;
Mukhopadhyay, Samrat
.
NATURE COMMUNICATIONS,
2022, 13 (01)

Avni, Anamika
论文数: 0 引用数: 0
h-index: 0
机构:
Indian Inst Sci Educ & Res IISER Mohali, Ctr Prot Sci Design & Engn, Mohali, Punjab, India
Indian Inst Sci Educ & Res IISER Mohali, Dept Chem Sci, Mohali, Punjab, India Indian Inst Sci Educ & Res IISER Mohali, Ctr Prot Sci Design & Engn, Mohali, Punjab, India

Joshi, Ashish
论文数: 0 引用数: 0
h-index: 0
机构:
Indian Inst Sci Educ & Res IISER Mohali, Ctr Prot Sci Design & Engn, Mohali, Punjab, India
Indian Inst Sci Educ & Res IISER Mohali, Dept Biol Sci, Mohali, Punjab, India Indian Inst Sci Educ & Res IISER Mohali, Ctr Prot Sci Design & Engn, Mohali, Punjab, India

Walimbe, Anuja
论文数: 0 引用数: 0
h-index: 0
机构:
Indian Inst Sci Educ & Res IISER Mohali, Ctr Prot Sci Design & Engn, Mohali, Punjab, India
Indian Inst Sci Educ & Res IISER Mohali, Dept Biol Sci, Mohali, Punjab, India Indian Inst Sci Educ & Res IISER Mohali, Ctr Prot Sci Design & Engn, Mohali, Punjab, India

Pattanashetty, Swastik G.
论文数: 0 引用数: 0
h-index: 0
机构:
Indian Inst Sci Educ & Res IISER Mohali, Ctr Prot Sci Design & Engn, Mohali, Punjab, India
Indian Inst Sci Educ & Res IISER Mohali, Dept Biol Sci, Mohali, Punjab, India Indian Inst Sci Educ & Res IISER Mohali, Ctr Prot Sci Design & Engn, Mohali, Punjab, India

论文数: 引用数:
h-index:
机构:
[2]
Gemcitabine: A pharmacologic and clinical overview
[J].
Barton-Burke, M
.
CANCER NURSING,
1999, 22 (02)
:176-183

Barton-Burke, M
论文数: 0 引用数: 0
h-index: 0
机构: Consulting Serv Boston, Boston, MA USA
[3]
Apoptosis-induced mitochondrial dysfunction causes cytoplasmic lipid droplet formation
[J].
Boren, J.
;
Brindle, K. M.
.
CELL DEATH AND DIFFERENTIATION,
2012, 19 (09)
:1561-1570

Boren, J.
论文数: 0 引用数: 0
h-index: 0
机构:
Canc Res UK Cambridge Res Inst, Li Ka Shing Ctr, Cambridge CB2 0RE, England
Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England Canc Res UK Cambridge Res Inst, Li Ka Shing Ctr, Cambridge CB2 0RE, England

Brindle, K. M.
论文数: 0 引用数: 0
h-index: 0
机构:
Canc Res UK Cambridge Res Inst, Li Ka Shing Ctr, Cambridge CB2 0RE, England
Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England Canc Res UK Cambridge Res Inst, Li Ka Shing Ctr, Cambridge CB2 0RE, England
[4]
Cancer and apoptosis: The apoptotic activity of plant and marine natural products and their potential as targeted cancer therapeutics
[J].
Chaudhry, Gul-e-Saba
;
Akim, Abdah Md
;
Sung, Yeong Yik
;
Sifzizul, Tengku Muhammad Tengku
.
FRONTIERS IN PHARMACOLOGY,
2022, 13

Chaudhry, Gul-e-Saba
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Malaysia Terengganu, Inst Marine Biotechnol, Kuala Terengganu, Malaysia Univ Malaysia Terengganu, Inst Marine Biotechnol, Kuala Terengganu, Malaysia

Akim, Abdah Md
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Putra Malaysia, Fac Med & Hlth Sci, Dept Biomed Sci, Seri Kembangan, Malaysia Univ Malaysia Terengganu, Inst Marine Biotechnol, Kuala Terengganu, Malaysia

Sung, Yeong Yik
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Malaysia Terengganu, Inst Marine Biotechnol, Kuala Terengganu, Malaysia Univ Malaysia Terengganu, Inst Marine Biotechnol, Kuala Terengganu, Malaysia

论文数: 引用数:
h-index:
机构:
[5]
Quantitative volumetric Raman imaging of three dimensional cell cultures
[J].
Kallepitis, Charalambos
;
Bergholt, Mads S.
;
Mazo, Manuel M.
;
Leonardo, Vincent
;
Skaalure, Stacey C.
;
Maynard, Stephanie A.
;
Stevens, Molly M.
.
NATURE COMMUNICATIONS,
2017, 8

Kallepitis, Charalambos
论文数: 0 引用数: 0
h-index: 0
机构:
Imperial Coll London, Dept Mat, London SW7 2AZ, England
Imperial Coll London, Dept Bioengn, London SW7 2AZ, England
Imperial Coll London, Inst Biomed Engn, London SW7 2AZ, England Imperial Coll London, Dept Mat, London SW7 2AZ, England

Bergholt, Mads S.
论文数: 0 引用数: 0
h-index: 0
机构:
Imperial Coll London, Dept Mat, London SW7 2AZ, England
Imperial Coll London, Dept Bioengn, London SW7 2AZ, England
Imperial Coll London, Inst Biomed Engn, London SW7 2AZ, England Imperial Coll London, Dept Mat, London SW7 2AZ, England

Mazo, Manuel M.
论文数: 0 引用数: 0
h-index: 0
机构:
Imperial Coll London, Dept Mat, London SW7 2AZ, England
Imperial Coll London, Dept Bioengn, London SW7 2AZ, England
Imperial Coll London, Inst Biomed Engn, London SW7 2AZ, England Imperial Coll London, Dept Mat, London SW7 2AZ, England

Leonardo, Vincent
论文数: 0 引用数: 0
h-index: 0
机构:
Imperial Coll London, Dept Mat, London SW7 2AZ, England
Imperial Coll London, Dept Bioengn, London SW7 2AZ, England
Imperial Coll London, Inst Biomed Engn, London SW7 2AZ, England Imperial Coll London, Dept Mat, London SW7 2AZ, England

Skaalure, Stacey C.
论文数: 0 引用数: 0
h-index: 0
机构:
Imperial Coll London, Dept Mat, London SW7 2AZ, England
Imperial Coll London, Dept Bioengn, London SW7 2AZ, England
Imperial Coll London, Inst Biomed Engn, London SW7 2AZ, England Imperial Coll London, Dept Mat, London SW7 2AZ, England

Maynard, Stephanie A.
论文数: 0 引用数: 0
h-index: 0
机构:
Imperial Coll London, Dept Mat, London SW7 2AZ, England
Imperial Coll London, Dept Bioengn, London SW7 2AZ, England
Imperial Coll London, Inst Biomed Engn, London SW7 2AZ, England Imperial Coll London, Dept Mat, London SW7 2AZ, England

Stevens, Molly M.
论文数: 0 引用数: 0
h-index: 0
机构:
Imperial Coll London, Dept Mat, London SW7 2AZ, England
Imperial Coll London, Dept Bioengn, London SW7 2AZ, England
Imperial Coll London, Inst Biomed Engn, London SW7 2AZ, England Imperial Coll London, Dept Mat, London SW7 2AZ, England
[6]
Erk2 Phosphorylation of Drp1 Promotes Mitochondrial Fission and MAPK-Driven Tumor Growth
[J].
Kashatus, Jennifer A.
;
Nascimento, Aldo
;
Myers, Lindsey J.
;
Sher, Annie
;
Byrne, Frances L.
;
Hoehn, Kyle L.
;
Counter, Christopher M.
;
Kashatus, David F.
.
MOLECULAR CELL,
2015, 57 (03)
:537-551

Kashatus, Jennifer A.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Virginia Hlth Syst, Dept Microbiol Immunol & Canc Biol, Charlottesville, VA 22908 USA Univ Virginia Hlth Syst, Dept Microbiol Immunol & Canc Biol, Charlottesville, VA 22908 USA

Nascimento, Aldo
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Virginia Hlth Syst, Dept Microbiol Immunol & Canc Biol, Charlottesville, VA 22908 USA Univ Virginia Hlth Syst, Dept Microbiol Immunol & Canc Biol, Charlottesville, VA 22908 USA

Myers, Lindsey J.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Virginia Hlth Syst, Dept Microbiol Immunol & Canc Biol, Charlottesville, VA 22908 USA Univ Virginia Hlth Syst, Dept Microbiol Immunol & Canc Biol, Charlottesville, VA 22908 USA

Sher, Annie
论文数: 0 引用数: 0
h-index: 0
机构:
Duke Univ, Med Ctr, Dept Radiat Oncol, Dept Pharmacol & Canc Biol, Durham, NC 27710 USA Univ Virginia Hlth Syst, Dept Microbiol Immunol & Canc Biol, Charlottesville, VA 22908 USA

Byrne, Frances L.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Virginia Hlth Syst, Dept Pharmacol, Charlottesville, VA 22908 USA Univ Virginia Hlth Syst, Dept Microbiol Immunol & Canc Biol, Charlottesville, VA 22908 USA

Hoehn, Kyle L.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Virginia Hlth Syst, Dept Pharmacol, Charlottesville, VA 22908 USA Univ Virginia Hlth Syst, Dept Microbiol Immunol & Canc Biol, Charlottesville, VA 22908 USA

Counter, Christopher M.
论文数: 0 引用数: 0
h-index: 0
机构:
Duke Univ, Med Ctr, Dept Radiat Oncol, Dept Pharmacol & Canc Biol, Durham, NC 27710 USA Univ Virginia Hlth Syst, Dept Microbiol Immunol & Canc Biol, Charlottesville, VA 22908 USA

Kashatus, David F.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Virginia Hlth Syst, Dept Microbiol Immunol & Canc Biol, Charlottesville, VA 22908 USA Univ Virginia Hlth Syst, Dept Microbiol Immunol & Canc Biol, Charlottesville, VA 22908 USA
[7]
Pancreatic cancer epidemiology: understanding the role of lifestyle and inherited risk factors
[J].
Klein, Alison P.
.
NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY,
2021, 18 (07)
:493-502

Klein, Alison P.
论文数: 0 引用数: 0
h-index: 0
机构:
Johns Hopkins Sch Med, Dept Oncol, Sidney Kimmel Comprehens Ctr, Baltimore, MD 21205 USA Johns Hopkins Sch Med, Dept Oncol, Sidney Kimmel Comprehens Ctr, Baltimore, MD 21205 USA
[8]
A synthetic chalcone, 2′-hydroxy-2,3,5′-trimethoxychalcone triggers unfolded protein response-mediated apoptosis in breast cancer cells
[J].
Lee, Da Hyun
;
Jung, You Jung
;
Koh, Dongsoo
;
Lim, Yoongho
;
Lee, Young Han
;
Shin, Soon Young
.
CANCER LETTERS,
2016, 372 (01)
:1-9

Lee, Da Hyun
论文数: 0 引用数: 0
h-index: 0
机构:
Konkuk Univ, Dept Biol Sci, Coll Biol Sci & Biotechnol, Seoul 05029, South Korea Konkuk Univ, Dept Biol Sci, Coll Biol Sci & Biotechnol, Seoul 05029, South Korea

Jung, You Jung
论文数: 0 引用数: 0
h-index: 0
机构:
Konkuk Univ, Canc & Metab Inst, Seoul 05029, South Korea Konkuk Univ, Dept Biol Sci, Coll Biol Sci & Biotechnol, Seoul 05029, South Korea

Koh, Dongsoo
论文数: 0 引用数: 0
h-index: 0
机构:
Dongduk Womens Univ, Dept Appl Chem, Seoul 02748, South Korea Konkuk Univ, Dept Biol Sci, Coll Biol Sci & Biotechnol, Seoul 05029, South Korea

论文数: 引用数:
h-index:
机构:

Lee, Young Han
论文数: 0 引用数: 0
h-index: 0
机构:
Konkuk Univ, Dept Biol Sci, Coll Biol Sci & Biotechnol, Seoul 05029, South Korea
Konkuk Univ, Canc & Metab Inst, Seoul 05029, South Korea Konkuk Univ, Dept Biol Sci, Coll Biol Sci & Biotechnol, Seoul 05029, South Korea

Shin, Soon Young
论文数: 0 引用数: 0
h-index: 0
机构:
Konkuk Univ, Dept Biol Sci, Coll Biol Sci & Biotechnol, Seoul 05029, South Korea
Konkuk Univ, Canc & Metab Inst, Seoul 05029, South Korea Konkuk Univ, Dept Biol Sci, Coll Biol Sci & Biotechnol, Seoul 05029, South Korea
[9]
Involvement of NF-κB and Bcl2/Bax signaling pathways in the apoptosis of MCF7 cells induced by a xanthone compound Pyranocycloartobiloxanthone A
[J].
Mohan, Syam
;
Abdelwahab, Siddig Ibrahim
;
Kamalidehghan, Behnam
;
Syam, Suvitha
;
May, Koh Sue
;
Harmal, Nabil Saad Mohammed
;
Shafifiyaz, Noor
;
Hadi, A. Hamid A.
;
Hashim, Najihah Mohd
;
Rahmani, Mawardi
;
Taha, Manal Mohamed Elhassan
;
Cheah, Shiau-Chuen
;
Zajmi, Asdren
.
PHYTOMEDICINE,
2012, 19 (11)
:1007-1015

Mohan, Syam
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia

Abdelwahab, Siddig Ibrahim
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia
Jazan Univ Jazan, Fac Med, Med Res Ctr, Jazan, Saudi Arabia Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia

Kamalidehghan, Behnam
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia

Syam, Suvitha
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia

May, Koh Sue
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia

Harmal, Nabil Saad Mohammed
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia

Shafifiyaz, Noor
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia

Hadi, A. Hamid A.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Malaya, Fac Sci, Kuala Lumpur 50603, Malaysia Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia

Hashim, Najihah Mohd
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia

Rahmani, Mawardi
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Putra Malaysia, Fac Sci, Dept Chem, Upm Serdang 43400, Malaysia Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia

Taha, Manal Mohamed Elhassan
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia

Cheah, Shiau-Chuen
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Malaya, Fac Med, Dept Pharmacol, Ctr Nat Prod & Drug Discovery CENAR, Kuala Lumpur 50603, Malaysia Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia

Zajmi, Asdren
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia
[10]
Bcl-2 inhibits Bax translocation from cytosol to mitochondria during drug-induced apoptosis of human tumor cells
[J].
Murphy, KM
;
Ranganathan, V
;
Farnsworth, ML
;
Kavallaris, M
;
Lock, RB
.
CELL DEATH AND DIFFERENTIATION,
2000, 7 (01)
:102-111

Murphy, KM
论文数: 0 引用数: 0
h-index: 0
机构: Childrens Canc Inst Australia Med Res, Randwick, NSW 2031, Australia

Ranganathan, V
论文数: 0 引用数: 0
h-index: 0
机构: Childrens Canc Inst Australia Med Res, Randwick, NSW 2031, Australia

Farnsworth, ML
论文数: 0 引用数: 0
h-index: 0
机构: Childrens Canc Inst Australia Med Res, Randwick, NSW 2031, Australia

Kavallaris, M
论文数: 0 引用数: 0
h-index: 0
机构: Childrens Canc Inst Australia Med Res, Randwick, NSW 2031, Australia

Lock, RB
论文数: 0 引用数: 0
h-index: 0
机构: Childrens Canc Inst Australia Med Res, Randwick, NSW 2031, Australia