Development of an animal-free nitrogen source for the liquid surface culture of Cordyceps militaris

被引:5
作者
Sripilai, Kritsana [1 ]
Chaicharoenaudomrung, Nipha [1 ]
Phonchai, Ruchee [1 ]
Chueaphromsri, Phongsakorn [1 ]
Kunhorm, Phongsakorn [1 ]
Noisa, Parinya [1 ]
机构
[1] Suranaree Univ Technol, Inst Agr Technol, Sch Biotechnol, Lab Cell Based Assays & Innovat, 111 Univ Ave, Suranari 30000, Nakhon Ratchasi, Thailand
关键词
Cordyceps militaris; animal-free nitrogen sources; cordycepin; vegetable seed; liquid surface culture; OPTIMIZATION; CARBON; CULTIVATION; ACIDS; OILS;
D O I
10.1093/lambio/ovad053
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Cordyceps militaris is a medicinal mushroom in Asia in the 21st century, which cordycepin is a significant bioactive compound. This study, investigated the effect of culture conditions and vegetable seed extract powder as a supplementary source of animal-free nitrogen on the production of cordycepin by C. militaris in liquid surface culture. The highest cordycepin production was observed under soybean extract powder (SBEP) conditions, and 80 g L-1 of SBEP supplementation increased cordycepin production to 2.52 g L-1, which was greater than the control (peptone). Quantitative polymerase chain reaction was used to examine the transcription levels, and the results showed that supplementing with SBEP 80 g L-1 significantly increased the expression of genes associated with the carbon metabolic pathway, amino acid metabolism, and two key genes involved in the cordycepin biosynthesis (cns1 and NT5E) compared to peptone-supplemented culture. Under optimal culture conditions, the model predicted a maximum response of cordycepin production of 2.64 g L-1 at a working volume of 147.5 ml, an inoculum size of 8.8% v/v, and a cultivation time of 40.0 days. This optimized culture condition could be used to increase cordycepin production in large-scale bioreactors. Additional research can be conducted to assess the economic viability of this process.
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页数:12
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