siRNA-Mediated Timp1 Silencing Inhibited the Inflammatory Phenotype during Acute Lung Injury

被引：10

作者：

Chernikov, Ivan V. ^{[1
]}

Staroseletz, Yaroslav Yu. ^{[1
]}

Tatarnikova, Irina S. ^{[1
]}

Sen'kova, Aleksandra V. ^{[1
]}

Savin, Innokenty A. ^{[1
]}

Markov, Andrey V. ^{[1
]}

Logashenko, Evgeniya B. ^{[1
]}

Chernolovskaya, Elena L. ^{[1
]}

Zenkova, Marina A. ^{[1
]}

Vlassov, Valentin V. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Chem Biol & Fundamental Med, Siberian Branch, Acad Lavrentiev Ave 8, Novosibirsk 630090, Russia

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2023年 / 24卷 / 02期

基金：

俄罗斯科学基金会;

关键词：

acute lung injury; inflammation; chemically modified siRNA; TIMP1; IL6; LPS; bioinformatics analysis; ACUTE RESPIRATORY-DISTRESS; TISSUE INHIBITOR; MATRIX METALLOPROTEINASES; TARGETING TIMP-1; TNF-ALPHA; DELIVERY; EXPRESSION; LIPOPOLYSACCHARIDE; PATHOGENESIS; ADAM8;

D O I：

10.3390/ijms24021641

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Acute lung injury is a complex cascade process that develops in response to various damaging factors, which can lead to acute respiratory distress syndrome. Within this study, based on bioinformatics reanalysis of available full-transcriptome data of acute lung injury induced in mice and humans by various factors, we selected a set of genes that could serve as good targets for suppressing inflammation in the lung tissue, evaluated their expression in the cells of different origins during LPS-induced inflammation, and chose the tissue inhibitor of metalloproteinase Timp1 as a promising target for suppressing inflammation. We designed an effective chemically modified anti-TIMP1 siRNA and showed that Timp1 silencing correlates with a decrease in the pro-inflammatory cytokine IL6 secretion in cultured macrophage cells and reduces the severity of LPS-induced acute lung injury in a mouse model.

引用

页数：27

共 132 条

[71] Targeted delivery of nucleic acids into xenograft tumors mediated by novel folate-equipped liposomes [J].

Kabilova, Tatyana O. ;

Shmendel, Elena V. ;

Gladkikh, Daniil V. ;

Chernolovskaya, Elena L. ;

Markov, Oleg V. ;

Morozova, Nina G. ;

Maslov, Mikhail A. ;

Zenkova, Marina A. .

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 2018, 123 :59-70

[72] Acute Respiratory Distress Syndrome: Etiology, Pathogenesis, and Summary on Management [J].

Kaku, Shawn ;

Nguyen, Christopher D. ;

Htet, Natalie N. ;

Tutera, Dominic ;

Barr, Juliana ;

Paintal, Harman S. ;

Kuschner, Ware G. .

JOURNAL OF INTENSIVE CARE MEDICINE, 2020, 35 (08) :723-737

[73] Gene regulation of the serine proteinase inhibitors α1-antitrypsin and α1-antichymotrypsin [J].

Kalsheker, N ;

Morley, S ;

Morgan, K .

BIOCHEMICAL SOCIETY TRANSACTIONS, 2002, 30 :93-98

[74] The Serpin Superfamily and Their Role in the Regulation and Dysfunction of Serine Protease Activity in COPD and Other Chronic Lung Diseases [J].

Kelly-Robinson, Gillian A. ;

Reihill, James A. ;

Lundy, Fionnuala T. ;

McGarvey, Lorcan P. ;

Lockhart, John C. ;

Litherland, Gary J. ;

Thornbury, Keith D. ;

Martin, S. Lorraine .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2021, 22 (12)

[75] Innate immune defense against pneumococcal pneumonia requires pulmonary complement component C3 [J].

Kerr, AR ;

Paterson, GK ;

Riboldi-Tunnicliffe, A ;

Mitchell, TJ .

INFECTION AND IMMUNITY, 2005, 73 (07) :4245-4252

[76] Mechanistic Understanding of Lung Inflammation: Recent Advances and Emerging Techniques [J].

Keskinidou, Chrysi ;

Vassiliou, Alice G. ;

Dimopoulou, ioanna ;

Kotanidou, Anastasia ;

Orfanos, Stylianos E. .

JOURNAL OF INFLAMMATION RESEARCH, 2022, 15 :3501-3546

[77] Tissue inhibitor of metalloproteinase-1 deficiency amplifies acute lung injury in bleomycin-exposed mice [J].

Kim, KH ;

Burkhart, K ;

Chen, P ;

Frevert, CW ;

Randolph-Habecker, J ;

Hackman, RC ;

Soloway, PD ;

Madtes, DK .

AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2005, 33 (03) :271-279

[78] Interleukin-6 regulation of matrix metalloproteinase (MMP-2 and MMP-9) and tissue inhibitor of metalloproteinase (TIMP-1) expression in malignant non-Hodgkin's lymphomas [J].

Kossakowska, AE ;

Edwards, DR ;

Prusinkiewicz, C ;

Zhang, MC ;

Guo, DL ;

Urbanski, SJ ;

Grogan, T ;

Marquez, LA ;

Janowska-Wieczorek, A .

BLOOD, 1999, 94 (06) :2080-2089

[79] Pulmonary delivery of therapeutic siRNA [J].

Lam, Jenny Ka-Wing ;

Liang, Wanling ;

Chan, Hak-Kim .

ADVANCED DRUG DELIVERY REVIEWS, 2012, 64 (01) :1-15

[80] The ITAM-bearing transmembrane adaptor DAP12 in lymphoid and myeloid cell function [J].

Lanier, LL ;

Bakker, ABH .

IMMUNOLOGY TODAY, 2000, 21 (12) :611-614

← 3 4 5 6 7 8 9 10 11 12 →