A Comparative Study of Voltammetric vs Impedimetric Immunosensor for Rapid SARS-CoV-2 Detection at the Point-of-care

被引：5

作者：

Tortolini, Cristina ^{[1
]}

Angeloni, Antonio ^{[1
]}

Antiochia, Riccarda ^{[2
]}

机构：

[1] Univ Roma La Sapienza, Dept Expt Med, Viale Regina Elena 324, I-00166 Rome, Italy

[2] Univ Roma La Sapienza, Dept Chem & Drug Technol, Ple Aldo Moro 5, I-00185 Rome, Italy

来源：

ELECTROANALYSIS | 2023年 / 35卷 / 04期

关键词：

Immunosensor; SARS-CoV-2; voltammetric biosensor; impedimetric biosensor; POC diagnostics; SARS-COV; CORONAVIRUS; BIOSENSOR; ASSAY;

D O I：

10.1002/elan.202200349

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Here, a novel biosensing platform for the detection of SARS-CoV-2 usable both at voltammetric and impedimetric mode is reported. The platform was constructed on a multi-walled carbon nanotubes (MWCNTs) screen-printed electrode (SPE) functionalized by methylene blue (MB), antibodies against SARS-CoV-2 spike protein (SP), a bioactive layer of chitosan (CS) and protein A (PrA). The voltammetric sensor showed superior performances both in phosphate buffer solution (PBS) and spiked-saliva samples, with LOD values of 5.0 +/- 0.1 and 30 +/- 2.1 ng/mL, compared to 20 +/- 1.8 and 50 +/- 2.5 ng/mL for the impedimetric sensor. Moreover, the voltammetric immunosensor was tested in real saliva, showing promising results.

引用

页数：10

共 55 条

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