Characterization and genomic analysis of a novel lytic phage vB_PstM_ZRG1 infecting Stutzerimonas stutzeri, representing a new viral genus, Elithevirus

被引：1

作者：

Chen, Ying ^{[1
]}

Guo, Ruizhe ^{[1
]}

Liang, Yantao ^{[1
,2
]}

Luo, Lin ^{[1
]}

Han, Ying ^{[1
]}

Wang, Hongmin ^{[1
]}

Zhang, Hong ^{[1
]}

Liu, Yundan ^{[1
]}

Zheng, Kaiyang ^{[1
]}

Shao, Hongbing ^{[1
,2
]}

Sung, Yeong Yik ^{[2
,3
]}

Mok, Wen Jye ^{[2
,3
]}

Wong, Li Lian ^{[2
,3
]}

Mcminn, Andrew ^{[1
,4
]}

Wang, Min ^{[1
,2
,5
,6
]}

机构：

[1] Ocean Univ China, Frontiers Sci Ctr Deep Ocean Multispheres & Earth, Ctr Ocean Carbon Neutral, Coll Marine Life Sci,Inst Evolut & Marine Biodiver, Qingdao, Peoples R China

[2] UMT OUC Joint Acad Ctr Marine Studies, Qingdao, Peoples R China

[3] Univ Malaysia Terengganu, Inst Marine Biotechnol, Kuala Terengganu, Malaysia

[4] Univ Tasmania, Inst Marine & Antarctic Studies, Hobart, Tas, Australia

[5] Ocean Univ China, Haide Coll, Qingdao, Peoples R China

[6] Qingdao Univ, Affiliated Hosp, Qingdao 266000, Peoples R China

来源：

VIRUS RESEARCH | 2023年 / 334卷

基金：

中国国家自然科学基金;

关键词：

Stutzerimonas stutzeri phage; Genomic analysis; Auxiliary metabolic genes; Phylogenetic analysis; Ecological distribution;

D O I：

10.1016/j.virusres.2023.199183

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Stutzerimonas stutzeri is an opportunistic pathogen widely distributed in the environment and displays diverse metabolic capabilities. In this study, a novel lytic S. stutzeri phage, named vB_PstM_ZRG1, was isolated from the seawater in the East China Sea (29 degrees 09'N, 123 degrees 39'E). vB_PstM_ZRG1 was stable at temperatures ranging from-20 degrees C to 65 degrees C and across a wide range of pH values from 3 to 10. The genome of vB_PstM_ZRG1 was determined to be a double-stranded DNA with a genome size of 52,767 bp, containing 78 putative open reading frames (ORFs). Three auxiliary metabolic genes encoded by phage vB_PstM_ZRG1 were predicted, including Toll/ interleukin-1 receptor (TIR) domain, proline-alanine-alanine-arginine (PAAR) protein and SGNH (Ser-Gly-Asn-His) family hydrolase, especially TIR domain is not common in isolated phages. Phylogenic and network analysis showed that vB_PstM_ZRG1 has low similarity to other phage genomes in the GenBank and IMG/VR database, and might represent a novel viral genus, named Elithevirus. Additionally, the distribution map results indicated that vB_PstM_ZRG1 could infect both extreme colds-and warm-type hosts in the marine environment. In summary, our finding provided basic information for further research on the relationship between S. stutzeri and their phages, and expanded our understanding of genomic characteristics, phylogenetic diversity and distribution of Elithevirus.

引用

页数：9

共 60 条

[1] The SGNH hydrolase family: a template for carbohydrate diversity [J].