Nitrogen application influences the effect of bacteria on the belowground allocation of photosynthesized carbon under elevated CO2

被引:16
作者
Jia, Weina [1 ,2 ]
Zheng, Tiantian [1 ]
Zhao, Yu [4 ]
Deng, Fangbo [1 ]
Yang, Yali [1 ]
Liang, Chao [1 ,3 ]
He, Hongbo [1 ,3 ]
Zhang, Xudong [1 ,3 ]
机构
[1] Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[3] Chinese Acad Sci, Inst Appl Ecol, Key Lab Conservat Tillage & Ecol Agr, Shenyang 110016, Liaoning, Peoples R China
[4] Dalian Minzu Univ, Coll Environm & Resources, Dalian 116600, Peoples R China
基金
中国博士后科学基金; 中国国家自然科学基金;
关键词
Elevated CO 2; N fertilization; Bacterial composition; Keystone taxa; Photosynthesized C sequestration; SOIL MICROBIAL COMMUNITIES; ORGANIC-MATTER DECOMPOSITION; ATMOSPHERIC CO2; STOMATAL CONDUCTANCE; ANALYSIS REVEALS; FERTILIZATION; SHIFTS; AVAILABILITY; GRASSLAND; RESPONSES;
D O I
10.1016/j.soilbio.2023.109021
中图分类号
S15 [土壤学];
学科分类号
0903 ; 090301 ;
摘要
Elevated carbon dioxide (eCO2) affects the translocation of photosynthesized carbon (C) from plants to soil, which is predominantly executed by soil microorganisms. However, the response strategy and mediation of the bacterial community on photosynthesized C allocation belowground remains elusive under eCO2 coupled with nitrogen (N) availability. Here, the spring wheat (Triticum aestivum L.) was continuously labeled with 13CO2 under ambient (aCO2, 350 ppm) or elevated (eCO2, 600 ppm) at three N application levels, and the incorporation of 13C into soil organic C was measured to indicate photosynthesized C accrual in soil. High-throughput MiSeq sequencing of 16S rRNA gene amplicons was used to explore soil bacterial response strategy. Compared with aCO2, eCO2 increased the relative abundance of Chloroflexi and Nitrospirae at the lowest N application level, while it increased that of Bacteroidetes at the highest N level. This shift of the response phyla from oligotrophic to copiotrophic species was closely associated with the increase in root biomass and its decomposability under eCO2 coupled with increasing N availability. The keystone taxa in the bacterial network might have strong ability to regulate such response of bacterial phyla to C and N availability. Specifically, the lower decomposability of the root-derived C caused by the intensive N deficiency under eCO2 stimulated oligotrophic bacteria, Saccharibacteria, Chloroflexi and Nitrospirae as keystone phyla, which could denote that the decomposition ability on recalcitrant substrate of bacterial phyla was enhanced before the activation of N transformation-related bacteria. Comparatively, sufficient N application under eCO2 facilitated copiotrophic bacteria as keystones for utilizing labile C derived from wheat roots, and thus the translocation efficiency of photosynthesized C belowground decreased. Our study sheds new light on the functional traits and mechanisms of the bacterial community in regulating C and N stoichiometry under eCO2.
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页数:9
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共 86 条
[1]   Microbial Hub Taxa Link Host and Abiotic Factors to Plant Microbiome Variation [J].
Agler, Matthew T. ;
Ruhe, Jonas ;
Kroll, Samuel ;
Morhenn, Constanze ;
Kim, Sang-Tae ;
Weigel, Detlef ;
Kemen, Eric M. .
PLOS BIOLOGY, 2016, 14 (01)
[2]   Reduced dependence of rhizosphere microbiome on plant-derived carbon in 32-year long-term inorganic and organic fertilized soils [J].
Ai, Chao ;
Liang, Guoqing ;
Sun, Jingwen ;
Wang, Xiubin ;
He, Ping ;
Zhou, Wei ;
He, Xinhua .
SOIL BIOLOGY & BIOCHEMISTRY, 2015, 80 :70-78
[3]   What have we learned from 15 years of free-air CO2 enrichment (FACE)?: A meta-analytic review of the responses of photosynthesis, canopy [J].
Ainsworth, EA ;
Long, SP .
NEW PHYTOLOGIST, 2005, 165 (02) :351-371
[4]   The response of photosynthesis and stomatal conductance to rising [CO2]:: mechanisms and environmental interactions [J].
Ainsworth, Elizabeth A. ;
Rogers, Alistair .
PLANT CELL AND ENVIRONMENT, 2007, 30 (03) :258-270
[5]   Shifts between Nitrospira- and Nitrobacter-like nitrite oxidizers underlie the response of soil potential nitrite oxidation to changes in tillage practices [J].
Attard, E. ;
Poly, F. ;
Commeaux, C. ;
Laurent, F. ;
Terada, A. ;
Smets, B. F. ;
Recous, S. ;
Le Roux, X. .
ENVIRONMENTAL MICROBIOLOGY, 2010, 12 (02) :315-326
[6]   Agricultural intensification reduces microbial network complexity and the abundance of keystone taxa in roots [J].
Banerjee, Samiran ;
Walder, Florian ;
Buechi, Lucie ;
Meyer, Marcel ;
Held, Alain Y. ;
Gattinger, Andreas ;
Keller, Thomas ;
Charles, Raphael ;
van der Heijden, Marcel G. A. .
ISME JOURNAL, 2019, 13 (07) :1722-1736
[7]   Keystone taxa as drivers of microbiome structure and functioning [J].
Banerjee, Samiran ;
Schlaeppi, Klaus ;
van der Heijden, Marcel G. A. .
NATURE REVIEWS MICROBIOLOGY, 2018, 16 (09) :567-576
[8]   Network analysis reveals functional redundancy and keystone taxa amongst bacterial and fungal communities during organic matter decomposition in an arable soil [J].
Banerjee, Samiran ;
Kirkby, Clive A. ;
Schmutter, Dione ;
Bissett, Andrew ;
Kirkegaard, John A. ;
Richardson, Alan E. .
SOIL BIOLOGY & BIOCHEMISTRY, 2016, 97 :188-198
[9]  
Bastian M., 2009, INT AAAI C WEBLOGS S, V3, P361
[10]   CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].
BENJAMINI, Y ;
HOCHBERG, Y .
JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300