Controlled Self-Assembly of Vesicles by Electrospray Deposition

The self-assembly of amphiphilic molecules produces structures of diverse dimensions, encompassing micelles, tubules, lamellae, and vesicles. This study focuses on elucidating the controllability of amphiphilic lipid molecule self-assembly in size and uniformity to facilitate our understanding of the molecular characteristics that correlate with the functions and attributes of the assembled structures. Electrospray deposition allows micropatterning of lipid molecules in conjunction with a conductive-nonconductive patterned substrate. The solvent in the sprayed mist undergoes evaporation during flight, leading to the deposition of dry lipids exclusively on the conductive regions of the substrate. This process enables homogeneous lipid micropatterning, effectively circumventing the coffee-ring effect. Subsequent hydration of the lipid pattern triggers the spontaneous formation of a size-controlled, unilamellar vesicle array on the substrate, spanning an area of a few square millimeters. The vesicles exhibits monodispersity, with a coefficient of variation below 8% for sizes ranging from 5 to 20 mu m. The size-controlled self-assembly process is adaptable to various lipid compositions, thereby demonstrating that the molecular characteristics manifest in the morphological features appear as phase separation, budding, and curvature of vesicle membranes. The approach further validates its suitability for conducting time-resolved analyses of molecular transport and ligand binding on the monodispersed vesicle array. This study focuses on controlling the self-assembly of lipid molecules. Using electrospray deposition, lipids are patterned on a conductive-nonconductive substrate, enabling homogeneous micropatterning. Subsequent hydration generates size-controlled, monodispersed vesicles with various lipid mixtures, demonstrating the correlation between molecular characteristics and morphological features. The approach is suitable for time-resolved analyses of molecular transport and ligand binding on the vesicle array.image (c) 2024 WILEY-VCH GmbH