Frequency of Extended-Spectrum Beta-Lactamases-Producing Escherichia coli Among Out- and In-patients in Rafsanjan City, Iran

被引:0
作者
Zarandi, Ebrahim Rezazadeh [1 ,2 ]
Hakimi, Hamid [1 ,2 ]
Bahramabadi, Reza [1 ,2 ]
Zare-Bidaki, Mohammad [1 ,2 ]
Ramezani, Mahnaz [1 ]
Shojaei, Ali [3 ]
Khodayari, Ali [3 ]
Fatemipour, Maryam [4 ]
Khademalhosseini, Morteza [4 ]
Assar, Shokrollah [2 ,5 ,6 ]
机构
[1] Rafsanjan Univ Med Sci, Res Inst Basic Med Sci, Immunol Infect Dis Res Ctr, Rafsanjan, Iran
[2] Rafsanjan Univ Med Sci, Sch Med, Dept Microbiol, Rafsanjan, Iran
[3] Rafsanjan Univ Med Sci, Rafsanjan, Iran
[4] Rafsanjan Univ Med Sci, Sch Med, Dept Pathol, Rafsanjan, Iran
[5] Rafsanjan Univ Med Sci, Geriatr Care Res Ctr, Rafsanjan, Iran
[6] Rafsanjan Univ Med Sci, Sch Med, Pistachio Co Ave, Rafsanjan 7718175911, Iran
关键词
Extended-spectrum Beta-Lactamase; Antibiotic Resistance Gene; Escherichia coli; In-patient; Out-patient; ANTIBIOTIC-RESISTANCE; KLEBSIELLA-PNEUMONIAE; EPIDEMIOLOGY; EVOLUTION; GENES; ESBLS; PREVALENCE; CTX-M-14; TEM;
D O I
10.5812/jjm-133216
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background: The frequency of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains among clinical isolates has been steadily increasing, leading to limited treatment options. Objectives: This study aimed to assess the antibiotic sensitivity of E. coli and the frequency of ESBL isolates among both out-patients and in-patients. Methods: A total of 390 E. coli isolates were received at the Ali-Ebn-e-Abitaleb Hospital laboratory in Rafsanjan. The antibiogram, as well as the phenotypic and genotypic detection of ESBL isolates, were conducted using Kirby-Bauer, combination disk confirmatory, and polymerase chain reaction tests, respectively. Results: Of all the E. coli isolates, 45.6% exhibited ESBL production. Among these isolates, 46.1% were obtained from hospital wards, while 42.5% were from outpatients. Meropenem and imipenem displayed sensitivities of 97.2% and 93.3%, respectively, whereas amikacin and nitrofurantoin showed sensitivities of 89.7% and 85.6%, respectively, for all isolates. Notably, ceftriaxone, cefotaxime, cefixime, ampicillin, co-trimoxazole, and nalidixic acid demonstrated high resistance rates, surpassing 50%. ESBL-producing isolates were more frequently observed in blood samples (65%) and wounds (60%) compared to other tested isolates. Approximately 8.6% of isolates carried a single type of ESBL gene, while 38.5% carried multiple ESBL genes. Conclusions: The data indicate a prevalence of ESBL-producing E. coli isolates among both out-patients and in-patients, with some of them acquiring two or three types of ESBL enzymes. As a result, their ability to hydrolyze antibiotics has increased, leading to their higher occurrence in clinical samples.
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页数:7
相关论文
共 29 条
[11]  
Fazeli Hossein, 2018, Adv Biomed Res, V7, P69, DOI 10.4103/abr.abr_164_17
[12]  
Fils LM, 2019, AFR J MICROBIOL RES, V13, P158, DOI [10.5897/ajmr2018.9042, DOI 10.5897/AJMR2018.9042]
[13]   Evolution and epidemiology of extended-spectrum β-lactamases (ESBLs) and ESBL-producing microorganisms [J].
Gniadkowski, M .
CLINICAL MICROBIOLOGY AND INFECTION, 2001, 7 (11) :597-608
[14]  
Goudarzi G., 2015, Pejouhandeh, V20, P33
[15]   Bloodstream infection with extended-spectrum beta-lactamase-producing Escherichia coli: The role of virulence genes [J].
Hung, Wan-Ting ;
Cheng, Ming-Fang ;
Tseng, Fan-Chen ;
Chen, Yao-Shen ;
Lee, Susan Shin-Jung ;
Chang, Tsung-Hsien ;
Lin, Hsi-Hsun ;
Hung, Chih-Hsin ;
Wang, Jiun-Ling .
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION, 2019, 52 (06) :947-955
[16]   Extensive drug-resistance in strains of Escherichia coli and Klebsiella pneumoniae isolated from paediatric urinary tract infections [J].
Iqbal, Zakia ;
Mumtaz, Muhammad Z. ;
Malik, Arif .
JOURNAL OF TAIBAH UNIVERSITY MEDICAL SCIENCES, 2021, 16 (04) :565-574
[17]   Detection of bla(TEM), bla(CTX-M), bla(CMY), and bla(SHV) Genes Among Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolated from Migratory Birds Travelling to Bangladesh [J].
Islam, Md Saiful ;
Sobur, Md Abdus ;
Rahman, Saifur ;
Ballah, Fatimah Mohammed ;
Ievy, Samina ;
Siddique, Mahbubul Pratik ;
Rahman, Marzia ;
Kafi, Md Abdul ;
Rahman, Md Tanvir .
MICROBIAL ECOLOGY, 2022, 83 (04) :942-950
[18]  
Kalantar D, 2010, JUNDISHAPUR J MICROB, V3, P137
[19]   Risk Factors of Extended-Spectrum Beta-Lactamases-Producing Escherichia coli Community Acquired Urinary Tract Infections: A Systematic Review [J].
Larramendy, Stephanie ;
Deglaire, Valentine ;
Dusollier, Paul ;
Fournier, Jean-Pascal ;
Caillon, Jocelyne ;
Beaudeau, Francois ;
Moret, Leila .
INFECTION AND DRUG RESISTANCE, 2020, 13 :3945-3955
[20]   Community-Acquired Urinary Tract Infection by Escherichia coli in the Era of Antibiotic Resistance [J].
Lee, Dong Sup ;
Lee, Seung-Ju ;
Choe, Hyun-Sop .
BIOMED RESEARCH INTERNATIONAL, 2018, 2018