In-vitro antioxidant and anti-inflammatory potential along with p.o. pharmacokinetic profile of key bioactive phytocompounds of Snow Mountain Garlic: a comparative analysis vis-à-vis normal garlic

被引：1

作者：

Kaur, Bhupinder ^{[1
,2
]}

Kumar, Nitish ^{[1
]}

Kumari, Laxmi ^{[2
]}

Gupta, Ajai P. ^{[3
]}

Sharma, Rajni ^{[1
]}

Chopra, Kanwaljit ^{[2
]}

Saxena, Shweta ^{[1
]}

机构：

[1] Minist Def, Def R&D Org DRDO, Def Inst High Altitude Res DIHAR, Med & Aromat Plant Div, C-O 56 APO, Leh 901205, Ladakh, India

[2] Panjab Univ, Fac Pharmaceut Sci, Univ Inst Pharmaceut Sci, Chandigarh 160014, India

[3] Minist Hlth & Family Welf, Qual Assurance, Food Safety & Stand Author India, New Delhi 110002, India

来源：

INFLAMMOPHARMACOLOGY | 2024年 / 32卷 / 03期

关键词：

Snow mountain garlic; Oxidative stress; S-Allyl-L-cysteine; LC-MS/MS-MRM; Immunomodulatory; Pharmacokinetics; ALLYL-L-CYSTEINE; NITRIC-OXIDE PRODUCTION; RAW; 264.7; MACROPHAGES; ALLIUM-SATIVUM; METABOLISM; EFFICACY; RATS;

D O I：

10.1007/s10787-024-01435-w

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Snow mountain garlic (SMG) is a trans-Himalayan medicinal plant used in the traditional medicine system for several ailments, including inflammatory arthritis. Research studies are insufficient to validate its folk medicinal applications. In the present study, the comparative abundance of its key bioactive phytocompounds, viz., S-allyl-L-cysteine (SAC), alliin, and S-methyl-L-cysteine (SMC) against normal garlic were assessed using the LC-MS/MS-MRM method. In addition, the study also explored the antioxidant and anti-inflammatory potency of crude extract of SMG and purified signature phytocompounds (i.e., SMC, SAC, and alliin) in comparison with normal garlic and dexamethasone in LPS-stimulated RAW264.7 macrophage cells. The LC-MS/MS-MRM study revealed significant differences among SMG and normal garlic, viz., alliin 22.8-fold higher in SMG, and SMC could be detected only in SMG. In the bioassays, SMG extract and purified signature phytocompounds significantly downregulated oxidative damage in activated macrophages, boosting endogenous antioxidants' activity. SMG extract-treated macrophages significantly suppressed NF-kappa B expression and related inflammatory indicators such as cytokines, COX-2, iNOS, and NO. Notably, the observed anti-inflammatory and antioxidant bioactivities of SMG extract were comparable to signature phytocompounds and dexamethasone. In addition, SAC being uniformly found in SMG and normal garlic, its comparative pharmacokinetics was studied to validate the pharmacodynamic superiority of SMG over normal garlic. Significantly higher plasma concentrations (Cmax), half-life (t1/2), and area under curve (AUC) of SAC following SMG extract administration than normal garlic validated the proposed hypothesis. Thus, the abundance of bioactive phytocompounds and their better pharmacokinetics in SMG extract might be underlying its medicinal merits over normal garlic.

引用

页码：1871 / 1886

页数：16