Human Placental Mesenchymal Stem Cell-derived Exosomes in Combination with Hyperbaric Oxygen Synergistically Promote Recovery after Spinal Cord Injury in Rats

被引：29

作者：

Cheshmi, Hosna ^{[1
]}

Mohammadi, Hossein ^{[2
]}

Akbari, Mitra ^{[3
]}

Nasiry, Davood ^{[4
]}

Rezapour-Nasrabad, Rafat ^{[5
]}

Bagheri, Mahdi ^{[6
]}

Abouhamzeh, Beheshteh ^{[7
]}

Poorhassan, Mahnaz ^{[8
]}

Mirhoseini, Mehri ^{[4
]}

Mokhtari, Hossein ^{[4
]}

Akbari, Esmaeil ^{[9
]}

Raoofi, Amir ^{[10
]}

机构：

[1] Iran Univ Med Sci, Dept Treatment, Tehran, Iran

[2] Isfahan Univ Med Sci, Sch Adv Technol Med, Dept Bioimaging, Esfahan, Iran

[3] Guilan Univ Med Sci, Amiralmomenin Hosp, Sch Med, Dept Eye, Rasht, Iran

[4] Mazandaran Univ Med Sci, Amol Fac Paramed Sci, Dept Paramed, Sari 4615861468, Iran

[5] Shahid Beheshti Univ Med Sci, Sch Nursing & Midwifery, Dept Psychiat Nursing & Management, Tehran 5865272565, Iran

[6] Persian Gulf Univ, Fac Nano & Bio Sci & Technol, Dept Biol Sci & Technol, Bushehr 75169, Iran

[7] Aja Univ Med Sci, Trauma Res Ctr, Tehran, Iran

[8] Smart Univ Med Sci, Dept Artificial Intelligence, Tehran, Iran

[9] Mazandaran Univ Med Sci, Sch Med, Dept Physiol, Sari, Iran

[10] Sabzevar Univ Med Sci, Cellular & Mol Res Ctr, Sabzevar, Iran

来源：

NEUROTOXICITY RESEARCH | 2023年 / 41卷 / 05期

关键词：

Spinal cord injury; Human placenta; Mesenchymal stem cells; Exosomes; Hyperbaric oxygen; INTERLEUKIN-1; INFLAMMATION; THERAPY; EXTRACT; PROTEIN;

D O I：

10.1007/s12640-023-00649-0

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Spinal cord injury (SCI) is a critical medical condition during which sensorimotor function is lost. Current treatments are still unable to effectively improve these conditions, so it is important to pay attention to other effective approaches. Currently, we investigated the combined effects of human placenta mesenchymal stem cells (hPMSCs)-derived exosomes along with hyperbaric oxygen (HBO) in the recovery of SCI in rats. Ninety male mature Sprague-Dawley (SD) rats were allocated into five equal groups, including; sham group, SCI group, Exo group (underwent SCI and received hPMSCs-derived exosomes), HBO group (underwent SCI and received HBO), and Exo+HBO group (underwent SCI and received hPMSCs-derived exosomes plus HBO). Tissue samples at the lesion site were obtained for the evaluation of stereological, immunohistochemical, biochemical, molecular, and behavioral characteristics. Findings showed a significant increase in stereological parameters, biochemical factors (GSH, SOD, and CAT), IL-10 gene expression and behavioral functions (BBB and EMG Latency) in treatment groups, especially Exo+HBO group, compared to SCI group. In addition, MDA levels, the density of apoptotic cells and gliosis, as well as expression of inflammatory genes (TNF-alpha and IL-1 beta) were considerably reduced in treatment groups, especially Exo+HBO group, compared to SCI group. We conclude that co-administration of hPMSCs-derived exosomes and HBO has synergistic neuroprotective effects in animals undergoing SCI.

引用

页码：431 / 445

页数：15