miR-34a-FOXP1 Loop in Ovarian Cancer

被引：3

作者：

Dirimtekin, Esra ^{[1
]}

Mortoglou, Maria ^{[2
]}

Alavanda, Ceren ^{[1
,3
]}

Yemlahi, Asmaa Benomar ^{[2
]}

Ates, Esra Arslan ^{[4
]}

Guney, Ilter ^{[1
]}

Uysal-Onganer, Pinar ^{[2
]}

机构：

[1] Marmara Univ, Sch Med, Dept Med Genet, TR-34854 Istanbul, Turkiye

[2] Univ Westminster, Sch Life Sci, Canc Mech & Biomarkers Res Grp, London W1W 6UW, England

[3] Univ Hlth Sci, Van Training & Res Hosp, Dept Med Genet, TR-65170 Van, Turkiye

[4] Istanbul Univ Cerrahpasa, Cerrahpasa Fac Med, Dept Med Genet, TR-34098 Istanbul, Turkiye

来源：

ACS OMEGA | 2023年 / 8卷 / 30期

关键词：

DOWN-REGULATION; MIR-34; FAMILY; FOXP1; MICRORNAS; RISK; PROLIFERATION; EXPRESSION; ONCOGENE; TARGET; BRCA1;

D O I：

10.1021/acsomega.3c03867

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Ovarian cancer (OC) is the main cause of gynecologicalcancer mortalityin most developed countries. microRNA (miR) expression dysregulationhas been highlighted in human cancers, and miR-34a is found to bedownregulated and associated with inhibition of tumor growth and invasionin several malignancies, including OC. The winged helix transcriptionfactor forkhead box P1 (FOXP1) is reported as either an oncogene ortumor suppressor in various cancers. This study aimed to elucidatepotential clinical and biological associations of miR-34a and transcriptionfactor FOXP1 in OC. We investigated nine OC patients' bloodsamples and two OC cell lines (SKOV-3 and OVCAR-3) using quantitativereal-time reverse transcription polymerase chain reaction (RT-qPCR)to determine both miR-34a and FOXP1 expressions. We have found thatmiR-34a and FOXP1 are reversely correlated in both in vitro and invivo. Inhibition of miR-34a transiently led to upregulation of FOXP1mRNA expression and increased cellular invasion in vitro. Our dataindicate that miR-34a could be a potential biomarker for improvingthe diagnostic efficiency of OC, and miR-34a overexpression may reduceOC pathogenesis by targeting FOXP1.

引用

页码：27743 / 27750

页数：8

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