A Photoinduced Electron Transfer-Based Hypochlorite-Specific Fluorescent Probe for Selective Imaging of Proinflammatory M1 in a Rheumatoid Arthritis Model

被引：36

作者：

Baruah, Mousumi ^{[1
]}

Kwon, Haw-Young ^{[2
]}

Cho, Heewon ^{[3
]}

Chang, Young-Tae ^{[2
,4
]}

Samanta, Animesh ^{[1
]}

机构：

[1] Shiv Nadar Univ, Sch Nat Sci, Dept Chem, Mol Sensors & Therapeut MST Res Lab, Greater Noida 201314, Uttar Pradesh, India

[2] Inst Basic Sci IBS, Ctr Selfassembly & Complex, Pohang 37673, South Korea

[3] Pohang Univ Sci & Technol POSTECH, Sch Interdisciplinary Biosci & Bioengn, Pohang 37673, South Korea

[4] Pohang Univ Sci & Technol POSTECH, Dept Chem, Pohang 37673, South Korea

来源：

ANALYTICAL CHEMISTRY | 2023年 / 95卷 / 08期

关键词：

MACROPHAGE POLARIZATION; LIVING CELLS; INFLAMMATION;

D O I：

10.1021/acs.analchem.2c05218

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The differentiation of the distinct phenotypes of macrophages is essential for monitoring the stage of inflammatory diseases for accurate diagnosis and treatment. Recent studies revealed that the level of hypochlorite (OCl-) varies from activated M1 macrophages (killing pathogens) to M2 (resolution of inflammation) during inflammation. Thus, we developed a simple and efficient fluorescent probe for discriminating M1 from M0 and M2. Herein, fluorescent-based imaging is applied as an alternative to immunohistochemistry, which is challenging due to the tedious process and high cost. We developed a hypochloritespecific probe PMS-T to differentiate M1 and M2, employing a metabolism-oriented live-cell distinction. This probe enables the detection of inflammatory rheumatoid arthritis in an ex vivo mouse model. Thus, it can be a potential chemical tool for monitoring inflammatory diseases, including rheumatoid arthritis, that may overcome the existing barriers of immunohistochemistry.

引用

页码：4147 / 4154

页数：8

共 61 条

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