Holtiella tumoricola gen. nov. sp. nov., isolated from a human clinical sample

被引：1

作者：

Allen-Vercoe, Emma ^{[1
]}

Daigneault, Michelle C. ^{[1
]}

Vancuren, Sarah J. ^{[1
]}

Cochrane, Kyla ^{[1
]}

O'Neal, Lindsey L. ^{[2
]}

Sankaranarayanan, Krithivasan ^{[2
,3
]}

Lawson, Paul A. ^{[2
]}

机构：

[1] Univ Guelph, Dept Mol & Cellular Biol, Guelph, ON, Canada

[2] Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK USA

[3] Univ Oklahoma, Labs Mol Anthropol & Microbiome Res, Norman, OK 73019 USA

来源：

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY | 2023年 / 73卷 / 07期

关键词：

16S rRNA; average amino acid identity; Cellulosilyticum; genome; taxonomy; FECAL MICROBIOTA; CLUSTAL-W; SEQUENCES; BACTERIA; DATABASE; DISEASE; CANCER;

D O I：

10.1099/ijsem.0.005958

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The diversity of bacteria associated with biopsy material obtained from patients with colorectal cancer was investigated using culture techniques. A novel bacterium, strain CC70AT, was isolated by diluting a sample of homogenized tissue in anaerobic medium, and then plating to yield a pure culture. Strain CC70AT was a Gram-positive, strictly anaerobic, motile, rod-shaped bac-terium. Formate, but not acetate, was a fermentative end-product from growth in peptone-yeast extract and peptone-yeast- glucose broth. The G+C content of DNA from strain CC70AT was 34.9 mol%. 16S rRNA gene sequence analysis revealed that the isolate was part of the phylum Bacillota. The closest described relatives of strain CC70AT were Cellulosilyticum lentocellum (93.3%) and Cellulosilyticum ruminicola (93.3 and 91.9% sequence similarity across 16S rRNA gene, respectively). According to the data obtained in this work, strain CC70AT represents a novel bacterium belonging to a new genus for which the name Holtiella tumoricola gen. nov., sp. nov. is proposed. The type strain for our described novel species is CC70AT (=DSM 27931T= JCM 30568T).

引用

页数：7

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