Tumor lineage-specific immune response in brain metastatic disease: opportunities for targeted immunotherapy regimen?

被引：4
作者：
Najjary, Shiva [1 ]
Kros, Johan M. M. [1 ]
de Koning, Willem [1 ]
Vadgama, Disha [1 ]
Lila, Karishma [2 ]
Wolf, Janina [2 ,3 ]
Mustafa, Dana A. M. [1 ]
机构：
[1] Erasmus Univ, Med Ctr, Dept Pathol & Clin Bioinformat, Tumor Immuno Pathol Lab, Dr Molewaterplein 40, NL-3015 GD Rotterdam, Netherlands
[2] Erasmus Univ, Med Ctr, Dept Pathol & Clin Bioinformat, Rotterdam, Netherlands
[3] Univ Bern, Inst Tissue Med & Pathol, Murten str 31, CH-3008 Bern, Switzerland
来源：
ACTA NEUROPATHOLOGICA COMMUNICATIONS | 2023年 / 11卷 / 01期
关键词：
Brain metastases; Lung adenocarcinoma; Breast cancer; Gene expression; Immune response; Immune infiltration; CELL LUNG-CANCER; MICROENVIRONMENT; EXPRESSION; DIAGNOSIS; SURVIVAL;
D O I：
10.1186/s40478-023-01542-9
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Metastases in the brain are the most severe and devastating complication of cancer. The incidence of brain metastasis is increasing. Therefore, the need of finding specific druggable targets for brain metastasis is demanding. The aim of this study was to compare the brain (immune) response to brain metastases of the most common tumor lineages, viz., lung adenocarcinoma and breast cancer. Targeted gene expression profiles of 11 brain metastasis of lung adenocarcinoma (BM-LUAD) were compared to 11 brain metastasis of breast cancer (BCBM) using NanoString nCounter PanCancer IO 360 (TM) Panel. The most promising results were validated spatially using the novel GeoMx (TM) Digital Spatial Profiler (DSP) Technology. Additionally, Immune cell profiles and expression of drug targets were validated by multiplex immunohistochemistry. We found a more active immune response in BM-LUAD as compared to BCBM. In the BM-LUAD, 138 genes were upregulated as compared to BCBM (adj. p <= 0.05). Conversely, in BCBM 28 genes were upregulated (adj. p <= 0.05). Additionally, genes related to CD45 + cells, T cells, and cytotoxic T cells showed to be expressed higher in BM-LUAD compared to BCBM (adj. p = 0.01, adj. p = 0.023, adj. p = 0.023, respectively). The spatial quantification of the immune cells using the GeoMx DSP technique revealed the significantly higher quantification of CD14 and CD163 in tumor regions of BM-LUAD as compared to BCBM. Importantly, the immune checkpoint VISTA and IDO1 were identified as highly expressed in the BM-LUAD. Multiplex immunohistochemistry confirmed the finding and showed that VISTA is expressed mainly in BM-LUAD tumor cells, CD3 + cells, and to fewer levels in some microglial cells in BM-LUAD. This is the first report on differences in the brain immune response between metastatic tumors of different lineages. We found a far more extensive infiltration of immune cells in BM-LUAD as compared to BCBM. In addition, we found higher expression of VISTA and IDO1 in BM-LUAD. Taken together, targeted immune therapy should be considered to treat patients with BM-LUAD.
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页数：12
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