Antimicrobial activities of graphene oxide against biofilm and intracellular Staphylococcus aureus isolated from bovine mastitis

被引:11
作者
Saeed, Shamsaldeen Ibrahim [1 ,2 ]
Vivian, Liang [1 ]
Zalati, C. W. Salma C. W. [1 ]
Sani, Nani Izreen Mohd [1 ]
Aklilu, Erkihun [1 ]
Mohamad, Maizan [1 ]
Noor, An'Amt Mohamed [3 ]
Muthoosamy, Kasturi [4 ]
Kamaruzzaman, Nor Fadhilah [1 ]
机构
[1] Univ Malaysia Kelantan, Fac Vet Med, Nanotechnol Vet Med NanoVet Res Grp, Kelantan 16100, Pengkalan Chepa, Malaysia
[2] Univ Nyala, Fac Vet Sci, POB 155, Nyala, South Darfur St, Sudan
[3] Univ Malaysia Kelantan, Fac Bioengn & Technol, Jeli 17700, Malaysia
[4] Univ Nottingham Malaysia, Ctr Nanotechnol & Adv Mat, Nanotechnol Res Grp, Semenyih 43500, Selangor, Malaysia
关键词
Graphene oxide; Mastitis; Intracellular S; aureus; IN-VITRO; MECHANISMS; ANTIBACTERIAL; RESISTANCE; BACTERIA; TOXICITY;
D O I
10.1186/s12917-022-03560-6
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
BackgroundS. aureus is one of the causative agents of bovine mastitis. The treatment using conventional antimicrobials has been hampered due to the development of antimicrobial resistance and the ability of the bacteria to form biofilms and localize inside the host cells.ObjectivesHere, the efficacy of graphene oxide (GO), a carbon-based nanomaterial, was tested against the biofilms and intracellular S. aureus invitro. Following that, the mechanism for the intracellular antimicrobial activities and GO toxicities was elucidated.MethodsGO antibiofilm properties were evaluated based on the disruption of biofilm structure, and the intracellular antimicrobial activities were determined by the survival of S. aureus in infected bovine mammary cells following GO exposure. The mechanism for GO intracellular antimicrobial activities was investigated using endocytosis inhibitors. GO toxicity towards the host cells was assessed using a resazurin assay.ResultsAt 100 ug/mL, GO reduced between 30 and 70% of S. aureus biofilm mass, suggesting GO's ability to disrupt the biofilm structure. At 200 ug/mL, GO killed almost 80% of intracellular S. aureus, and the antimicrobial activities were inhibited when cells were pre-treated with cytochalasin D, suggesting GO intracellular antimicrobial activities were dependent on the actin-polymerization of the cell membrane. At < 250 ug/mL, GO enhanced the viability of the Mac-T cell, and cells were only affected at higher dosages.ConclusionThe in vitro efficacy of GO against S. aureus in vitro suggested the compound could be further tested in Vivo to zrecognize its potential as one of the components of bovine mastitis therapy.
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页数:11
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