A fluorescent biosensor based on carbon quantum dots and single-stranded DNA for the detection of Escherichia coli

被引：10

作者：

Bai, Xiaolian ^{[1
]}

Ga, Lu ^{[2
]}

Ai, Jun ^{[1
]}

机构：

[1] Inner Mongolia Normal Univ, Coll Chem & Environm Sci, Inner Mongolia Key Lab Green Catalysis, 81 Zhaowudalu, Hohhot 010022, Peoples R China

[2] Inner Mongolia Med Univ, Coll Pharm, Hohhot 010110, Peoples R China

来源：

ANALYST | 2023年 / 148卷 / 16期

基金：

中国国家自然科学基金;

关键词：

SELECTIVE DETECTION; ACRYLAMIDE; NANOPARTICLES; O157H7; HEMIN;

D O I：

10.1039/d3an01024d

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

To detect E. coli in food, a simple fluorescent biosensor based on single-stranded DNA (ssDNA) and carbon quantum dots (CQDs) was developed. The carbon quantum dots were synthesized using a superhydrothermal method with carrot juice as a carbon source. The fluorescence intensity of the CQDs was decreased by induced ssDNA attachment. In the presence of E. coli, ssDNA preferentially binds to E. coli through hydrogen bonding and its fluorescence is greater than that in the absence of E. coli. The results showed that the linear range of the sensor was 1 x 10(2)-1 x 10(8) CFU mL(-1) with a coefficient of determination (R-2) of 0.9870. The detection limit for E. coli was 60 CFU mL(-1).

引用

页码：3892 / 3898

页数：7

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