Human platelet lysate enhances proliferation but not chondrogenic differentiation of pediatric mesenchymal progenitors

被引:4
|
作者
Gardner, Oliver F. W. [1 ]
Agabalyan, Natacha [1 ]
Weil, Ben [2 ]
Ali, Mohammed H. I. [1 ,3 ]
Lowdell, Mark W. [2 ,4 ]
Bulstrode, Neil W. [5 ]
Ferretti, Patrizia [1 ,6 ]
机构
[1] UCL GOS Inst Child Hlth, Dev Biol & Canc Dept, London, England
[2] Royal Free London NHS Fdn Trust, Ctr Cell Gene & Tissue Therapeut, London, England
[3] South Valley Univ, Fac Sci, Dept Zool, Qena, Egypt
[4] UCL, Canc Inst, London, England
[5] Great Ormond St Hosp Children NHS Fdn Trust, Dept Plast Surg, London, England
[6] UCL Inst Child Hlth, Stem Cells & Regenerat Med Sect, 30 Guilford St, London WC1N 1EH, England
关键词
adipose-derived stem cells; auricular reconstruction; cartilage; cartilage stem; progenitor cells; chondrogenesis; human platelet lysate; spheroid; FETAL BOVINE SERUM; STROMAL CELLS; ADIPOSE-TISSUE; STEM-CELLS; ARTICULAR-CARTILAGE; IN-VITRO; EXPANSION; CHONDROCYTES; CAPACITY; PHENOTYPE;
D O I
10.1016/j.jcyt.2022.11.007
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Background aims: Cell therapies have the potential to improve reconstructive procedures for congenital cra-niofacial cartilage anomalies such as microtia. Adipose-derived stem cells (ADSCs) and auricular cartilage stem/progenitor cells (CSPCs) are promising candidates for cartilage reconstruction, but their successful use in the clinic will require the development of xeno-free expansion and differentiation protocols that can max-imize their capacity for chondrogenesis.Methods: We assessed the behavior of human ADSCs and CSPCs grown either in qualified fetal bovine serum (FBS) or human platelet lysate (hPL), a xeno-free alternative, in conventional monolayer and 3-dimensional spheroid cultures.Results: We show that CSPCs and ADSCs display greater proliferation rate in hPL than FBS and express typical mesenchymal stromal cell surface antigens in both media. When expanded in hPL, both cell types, particu-larly CSPCs, maintain a spindle-like morphology and lower surface area over more passages than in FBS. Both media supplements support chondrogenic differentiation of CSPCs and ADSCs grown either as monolayers or spheroids. However, chondrogenesis appears less ordered in hPL than FBS, with reduced co-localization of aggrecan and collagen type II in spheroids. Conclusions: hPL may be beneficial for the expansion of cells with chondrogenic potential and maintaining stemness, but not for their chondrogenic differentiation for tissue engineering or disease modeling.(c) 2022 International Society for Cell & Gene Therapy. Published by Elsevier Inc. All rights reserved.
引用
收藏
页码:286 / 297
页数:12
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