Nisin:Carboxymethylcellulose polyion complex (PIC) nanoparticles. Preparation and antimicrobial activity

被引:17
作者
Celen, Teyfik [1 ,2 ,3 ]
Anumudu, Christian [2 ]
Miri, Taghi [2 ]
Onyeaka, Helen [2 ]
Fernandez-Trillo, Paco [1 ,2 ,4 ,5 ]
机构
[1] Univ Birmingham, Sch Chem, Birmingham B15 2TT, England
[2] Univ Birmingham, Sch Chem Engn, Birmingham B15 2TT, England
[3] Univ Birmingham, Inst Microbiol & Infect, Birmingham B15 2TT, England
[4] Univ A Coruna, Fac Ciencias, Dept Quim, La Coruna 15071, Spain
[5] Univ A Coruna, Ctr Invest Cient Avanzadas CICA, La Coruna 15071, Spain
关键词
Biopreservation; Natural preservatives; Nisin; Sustained antimicrobial activity; Polyion complex nanoparticles; NISIN; STABILITY;
D O I
10.1016/j.carbpol.2023.121032
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Nisin is a cationic antimicrobial peptide used as a natural food preservative against gram-positive bacteria. However, nisin is degraded following interaction with food components. Here, we report the first use of Carboxymethylcellulose (CMC), a versatile and affordable food additive, to protect nisin and extend its antimicrobial activity. First, we optimized the methodology by considering the effect of nisin:CMC ratio, pH, and, especially, the degree of substitution of CMC. In particular, we show here how these parameters affected the size, charge, and, notably, the encapsulation efficiency of these nanomaterials. This way, optimized formulations contained over 60 % w/w in nisin while encapsulating similar to 90 % of the nisin used. We then show that these new nanomaterials inhibited the growth of Staphylococcus aureus, a major foodborne pathogen, using milk as a representative food matrix. Remarkably, this inhibitory effect was observed with one-tenth of the concentration of nisin currently used in dairy products. We believe that the combination of the affordability of CMC, flexibility and simplicity of preparation, and the ability to inhibit the growth of food pathogens, makes these nisin:CMC PIC nanoparticles an ideal platform to underpin new nisin formulations.
引用
收藏
页数:7
相关论文
共 32 条
[2]  
Benmechernene Zineb, 2013, Recent Pat DNA Gene Seq, V7, P66
[3]   Synthesis, characterization of nisin loaded alginate-chitosan-pluronic composite nanoparticles and evaluation against microbes [J].
Bernela, Manju ;
Kaur, Pawan ;
Chopra, Meenu ;
Thakur, Rajesh .
LWT-FOOD SCIENCE AND TECHNOLOGY, 2014, 59 (02) :1093-1099
[4]  
Boualem K., 2013, Journal of Food Research, V2, P36
[5]   Carboxymethyl cellulose with tailored degree of substitution obtained from bacterial cellulose [J].
Casaburi, Agustina ;
Montoya Rojo, Ursula ;
Cerrutti, Patricia ;
Vazquez, Analia ;
Laura Foresti, Maria .
FOOD HYDROCOLLOIDS, 2018, 75 :147-156
[6]   Surfactant assisted nisin loaded chitosan-carageenan nanocapsule synthesis for controlling food pathogens [J].
Chopra, Meenu ;
Kaur, Pawan ;
Bernela, Manju ;
Thakur, Rajesh .
FOOD CONTROL, 2014, 37 :158-164
[7]   Preparation and properties of gum arabic cross-link binding nisin microparticles [J].
Gong, Feng ;
Qian, Junqing ;
Chen, Yan ;
Yao, Shen ;
Tong, Jun ;
Guo, Hui .
CARBOHYDRATE POLYMERS, 2018, 197 :608-613
[8]  
Held P., 2001, FLUOROMETRIC QUANTIT
[9]   Invited review: Advances in nisin use for preservation of dairy products [J].
Ibarra-Sanchez, Luis A. ;
El-Haddad, Nancy ;
Mahmoud, Darine ;
Miller, Michael J. ;
Karam, Layal .
JOURNAL OF DAIRY SCIENCE, 2020, 103 (03) :2041-2052
[10]   Polyion complex (PIC) particles: Preparation and biomedical applications [J].
Insua, Ignacio ;
Wilkinson, Andrew ;
Fernandez-Trillo, Francisco .
EUROPEAN POLYMER JOURNAL, 2016, 81 :198-215