Derivation of human primordial germ cell-like cells in an embryonic-like culture

被引：17

作者：

Esfahani, Sajedeh Nasr ^{[1
]}

Zheng, Yi ^{[1
,14
]}

Arabpour, Auriana ^{[2
,3
,4
]}

Irizarry, Agnes M. Resto ^{[1
]}

Kobayashi, Norio ^{[1
]}

Xue, Xufeng ^{[1
]}

Shao, Yue ^{[5
]}

Zhao, Cheng ^{[6
]}

Agranonik, Nicole L. ^{[4
]}

Sparrow, Megan ^{[4
]}

Hunt, Timothy J. ^{[4
]}

Faith, Jared ^{[4
]}

Lara, Mary Jasmine ^{[4
]}

Wu, Qiu Ya ^{[4
]}

Silber, Sherman ^{[7
]}

Petropoulos, Sophie ^{[6
,8
,9
,10
]}

Yang, Ran ^{[11
]}

Chien, Kenneth R. ^{[11
]}

Clark, Amander T. ^{[2
,3
,4
]}

Fu, Jianping ^{[1
,12
,13
]}

机构：

[1] Univ Michigan, Dept Mech Engn, Ann Arbor, MI 48109 USA

[2] Univ Calif Los Angeles, Mol Biol Inst, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Eli & Edythe Broad Ctr Regenerat Med & Stem Cell R, Los Angeles, CA 90095 USA

[4] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90095 USA

[5] Tsinghua Univ, Inst Biomech & Med Engn, Sch Aerosp Engn, Dept Engn Mech, Beijing 100084, Peoples R China

[6] Karolinska Inst, Dept Clin Sci Intervent & Technol, Div Obstet & Gynecol, S-14186 Stockholm, Sweden

[7] St Lukes Hosp, Infertil Ctr St Louis, St. Louis, MO 63017 USA

[8] Univ Montreal, Dept Med, Montreal, PQ, Canada

[9] Ctr Hosp Univ Montreal, Ctr Rech, Axe Immunopathol, Montreal, PQ H2X 19A, Canada

[10] Univ Montreal, Dept Med, Mol Biol Programme, Montreal, PQ, Canada

[11] Karolinska Inst, Dept Cell & Mol Biol, S-17177 Stockholm, Sweden

[12] Univ Michigan, Med Sch, Dept Cell & Dev Biol, Ann Arbor, MI 48109 USA

[13] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA

[14] Syracuse Univ, Dept Biomed & Chem Engn, Syracuse, NY 13244 USA

来源：

NATURE COMMUNICATIONS | 2024年 / 15卷 / 01期

基金：

美国国家卫生研究院; 美国国家科学基金会; 瑞典研究理事会;

关键词：

LINEAGE; SPECIFICATION; PLURIPOTENCY; MONKEYS; FATE;

D O I：

10.1038/s41467-023-43871-2

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Primordial germ cells (PGCs) are the embryonic precursors of sperm and eggs. They transmit genetic and epigenetic information across generations. Given the prominent role of germline defects in diseases such as infertility, detailed understanding of human PGC (hPGC) development has important implications in reproductive medicine and studying human evolution. Yet, hPGC specification remains an elusive process. Here, we report the induction of hPGC-like cells (hPGCLCs) in a bioengineered human pluripotent stem cell (hPSC) culture that mimics peri-implantation human development. In this culture, amniotic ectoderm-like cells (AMLCs), derived from hPSCs, induce hPGCLC specification from hPSCs through paracrine signaling downstream of ISL1. Our data further show functional roles of NODAL, WNT, and BMP signaling in hPGCLC induction. hPGCLCs are successfully derived from eight non-obstructive azoospermia (NOA) participant-derived hPSC lines using this biomimetic platform, demonstrating its promise for screening applications. Derivation of human primordial germ cell-like cells (hPGCLCs) is critical for reproductive medicine. Here, authors report the induction of hPGCLCs in a bioengineered human pluripotent stem cell culture that mimics peri-implantation human development.

引用

页数：12

共 50 条

[21] Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids [J].

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[22] CRISPR loss of function screening to identify genes involved in human primordial germ cell-like cell development [J].

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[23] Primordial germ cell-like cells derived from canine adipose mesenchymal stem cells [J].

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[24] Cyclosporin A and FGF signaling support the proliferation/survival of mouse primordial germ cell-like cells in vitro [J].

Ohta, Hiroshi ;

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Murase, Yusuke ;

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Saitou, Mitinori .

BIOLOGY OF REPRODUCTION, 2021, 104 (02) :344-360

[25] Porcine Primordial Germ Cell-Like Cells Generated from Induced Pluripotent Stem Cells Under Different Culture Conditions [J].

Godoy Pieri, Naira Caroline ;

de Souza, Aline Fernanda ;

Botigelli, Ramon Cesar ;

de Figueiredo Pessoa, Lais Vicari ;

Recchia, Kaiana ;

Machado, Lucas Simoes ;

Gloria, Mayra Hirakawa ;

Guimaraes de Castro, Raquel Vasconcelos ;

Leal, Diego Feitosa ;

Neto, Paulo Fantinato ;

Massami Kitamura Martins, Simone Maria ;

Martins, Daniele dos Santos ;

Bressan, Fabiana Fernandes ;

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STEM CELL REVIEWS AND REPORTS, 2022, 18 (05) :1639-1656

[26] Primordial Germ Cell-Like Cells Differentiated In Vitro from Skin-Derived Stem Cells [J].

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Dyce, Paul ;

Li, Julang .

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[27] In vitro expansion of mouse primordial germ cell-like cells recapitulates an epigenetic blank slate [J].

Ohta, Hiroshi ;

Kurimoto, Kazuki ;

Okamoto, Ikuhiro ;

Nakamura, Tomonori ;

Yabuta, Yukihiro ;

Miyauchi, Hidetaka ;

Yamamoto, Takuya ;

Okuno, Yukiko ;

Hagiwara, Masatoshi ;

Shirane, Kenjiro ;

Sasaki, Hiroyuki ;

Saitou, Mitinori .

EMBO JOURNAL, 2017, 36 (13) :1888-1907

[28] Embryonic stem cell-like cells established by culture of adult ovarian cells in mice [J].

Gong, Seung Pyo ;

Lee, Seung Tae ;

Lee, Eun Ju ;

Kim, Dae Yong ;

Lee, Gene ;

Chi, Sung Gil ;

Ryu, Byung-Kyu ;

Lee, Chae Hyun ;

Yum, Kyung Eun ;

Lee, Ho-Joon ;

Han, Jae Yong ;

Tilly, Jonathan L. ;

Lim, Jeong Mook .

FERTILITY AND STERILITY, 2010, 93 (08) :2594-U153

[29] Murine and Human Very Small Embryonic-Like Cells: A Perspective [J].

Heider, Andreas ;

Danova-Alt, Ralitza ;

Egger, Dietmar ;

Cross, Michael ;

Alt, Ruediger .

CYTOMETRY PART A, 2013, 83A (01) :72-75

[30] Generation of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients [J].

de Souza, Aline Fernanda ;

Bressan, Fabiana Fernandes ;

Pieri, Naira Caroline Godoy ;

Botigelli, Ramon Cesar ;

Revay, Tamas ;

Haddad, Simone Kashima ;

Covas, Dimas Tadeu ;

Ramos, Ester Silveira ;

King, Willian Allan ;

Meirelles, Flavio Vieira .

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