Mild Photothermal Therapy Prevents Posterior Capsule Opacification through Cytoskeletal Remodeling

Cataract is the first leading cause of blindness in the world and posterior capsule opacification (PCO) is the most common long-term complication after surgery. The primary pathogenic processes contributing to PCO are the proliferation and migration of residual lens epithelial cells (LECs). This study aimed to explore the mild photothermal effect on LECs. Interestingly, this work finds that the mild photothermal effect significantly inhibited the proliferation and migration of LECs. The live cell fluorescence imaging reveals that the remodeling of the actin cytoskeleton and cell morphology attributed to the inhibition effect. Further mechanistic studies at molecular level suggest that the mild photothermal effect can regulate the phosphorylation of ERM, YAP, and Cofilin and thereby affect the proliferation and migration of LECs. In order to explore the potential clinical application of mild photothermal therapy for PCO prevention, PDA/PVA gel rings with photothermal effect is prepared by the repeated freeze-thaw method and conducted experiments in vivo, which achieved favorable PCO prevention effect. Overall, this study shows that the mild photothermal effect can regulate the proliferation and migration of LECs through cytoskeletal remodeling and the results of experiments in vivo demonstrate that mild photothermal effect is a promising approach for PCO prevention. Posterior capsule opacification (PCO) frequently arises as a complication following cataract surgery. Herein, a hydrogel that harnesses near-infrared activated mild photothermal properties by incorporating polydopamine (PDA) nanoparticles into a polyvinyl alcohol (PVA) framework is designed. The biocompatible PDA/PVA hydrogel mitigates PCO by remodeling the cytoskeleton of lens epithelial cells. This work presents a viable strategy for PCO prevention.image