Soybean Calmodulin-Binding Transcription Activators, GmCAMTA2 and GmCAMTA8, Coordinate the Circadian Regulation of Developmental Processes and Drought Stress Responses

被引：11

作者：

Baek, Dongwon ^{[1
]}

Cho, Hyun Min ^{[2
]}

Cha, Ye Jin ^{[2
]}

Jin, Byung Jun ^{[3
]}

Lee, Su Hyeon ^{[2
]}

Park, Mi Suk ^{[1
]}

Chun, Hyun Jin ^{[3
]}

Kim, Min Chul ^{[1
,2
,3
]}

机构：

[1] Gyeongsang Natl Univ, Plant Mol Biol & Biotechnol Res Ctr, Jinju 52828, South Korea

[2] Gyeongsang Natl Univ, Div Appl Life Sci BK21 Four, Jinju 52828, South Korea

[3] Gyeongsang Natl Univ, Inst Agr & Life Sci, Jinju 52828, South Korea

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2023年 / 24卷 / 14期

基金：

新加坡国家研究基金会;

关键词：

GmCAMTA; circadian rhythm; development; drought stress; DEFENSE RESPONSES; EXPRESSION; IDENTIFICATION; CALCIUM; CLOCK; GENES; TOLERANCE; ROLES;

D O I：

10.3390/ijms241411477

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The calmodulin-binding transcription activators (CAMTAs) mediate transcriptional regulation of development, growth, and responses to various environmental stresses in plants. To understand the biological roles of soybean CAMTA (GmCAMTA) family members in response to abiotic stresses, we characterized expression patterns of 15 GmCAMTA genes in response to various abiotic stresses. The GmCAMTA genes exhibited distinct circadian regulation expression patterns and were differently expressed in response to salt, drought, and cold stresses. Interestingly, the expression levels of GmCAMTA2, GmCAMTA8, and GmCAMTA12 were higher in stem tissue than in other soybean tissues. To determine the roles of GmCAMTAs in the regulation of developmental processes and stress responses, we isolated GmCAMTA2 and GmCAMTA8 cDNAs from soybean and generated Arabidopsis overexpressing transgenic plants. The GmCAMTA2-OX and GmCAMTA8-OX plants showed hypersensitivity to drought stress. The water in the leaves of GmCAMTA2-OX and GmCAMTA8-OX plants was lost faster than that in wild-type (WT) plants under drought-stress conditions. In addition, stress-responsive genes were down-regulated in the GmCAMTA2-OX and GmCAMTA8-OX plants under drought stress conditions compared to WT plants. Our results suggest that GmCAMTA2 and GmCAMTA8 genes are regulated by circadian rhythms and function as negative regulators in development and drought stress responses.

引用

页数：13

共 45 条

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