Antibacterial and Antibiofilm Activity of Ficus carica-Mediated Calcium Oxide (CaONPs) Phyto-Nanoparticles

被引:12
作者
Khan, Asif Ullah [1 ]
Hussain, Tahir [1 ]
Abdullah, Mubarak Ali [2 ,3 ]
Khan, Mubarak Ali M. [4 ]
Almostafa, Mervt S. [5 ]
Younis, Nancy [6 ]
Yahya, Galal [7 ]
机构
[1] Abdul Wali Khan Univ, Dept Microbiol, Mardan 23200, Pakistan
[2] Silesian Tech Univ, Dept Phys Chem & Technol Polymers, PL-44100 Gliwice, Poland
[3] Silesian Tech Univ, Joint Doctoral Sch, Akad 2A, PL-44100 Gliwice, Poland
[4] Abdul Wali Khan Univ, Dept Biotechnol, Mardan 23200, Pakistan
[5] King Faisal Univ, Coll Sci, Dept Chem, Al Hasa 31982, Saudi Arabia
[6] King Faisal Univ, Coll Clin Pharm, Dept Pharmaceut Sci, Al Hasa 31982, Saudi Arabia
[7] Zagazig Univ, Fac Pharm, Dept Microbiol & Immunol, Al Sharqia 44519, Egypt
来源
MOLECULES | 2023年 / 28卷 / 14期
关键词
nanoparticles; characterization; biomedical applications; antimicrobial; antibiofilm; GREEN SYNTHESIS;
D O I
10.3390/molecules28145553
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The significance of nanomaterials in biomedicines served as the inspiration for the design of this study. In this particular investigation, we carried out the biosynthesis of calcium oxide nanoparticles (CaONPs) by employing a green-chemistry strategy and making use of an extract of Ficus carica (an edible fruit) as a capping and reducing agent. There is a dire need for new antimicrobial agents due to the alarming rise in antibiotic resistance. Nanoparticles' diverse antibacterial properties suggest that they might be standard alternatives to antimicrobial drugs in the future. We describe herein the use of a Ficus carica extract as a capping and reducing agent in the phyto-mediated synthesis of CaONPs for the evaluation of their antimicrobial properties. The phyto-mediated synthesis of NPs is considered a reliable approach due to its high yield, stability, non-toxicity, cost-effectiveness and eco-friendliness. The CaONPs were physiochemically characterized by UV-visible spectroscopy, energy-dispersive X-ray (EDX), scanning-electron microscopy (SEM), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR). The biological synthesis of the calcium oxide nanoparticles revealed a characteristic surface plasmon resonance peak (SPR) at 360 nm in UV-Vis spectroscopy, which clearly revealed the successful reduction of the Ca2+ ions to Ca-0 nanoparticles. The characteristic FTIR peak seen at 767 cm(-1) corresponded to Ca-O bond stretching and, thus, confirmed the biosynthesis of the CaONPs, while the scanning-electron micrographs revealed near-CaO aggregates with an average diameter of 84.87 & PLUSMN; 2.0 nm. The antibacterial and anti-biofilm analysis of the CaONPs showed inhibition of bacteria in the following order: P. aeruginosa (28 & PLUSMN; 1.0) > S. aureus (23 & PLUSMN; 0.3) > K. pneumoniae (18 & PLUSMN; 0.9) > P. vulgaris (13 & PLUSMN; 1.6) > E. coli (11 & PLUSMN; 0.5) mm. The CaONPs were shown to considerably inhibit biofilm formation, providing strong evidence for their major antibacterial activity. It is concluded that this straightforward environmentally friendly method is capable of synthesizing stable and effective CaONPs. The therapeutic value of CaONPs is indicated by their potential as a antibacterial and antibiofilm agents in future medications.
引用
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页数:13
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