Isopimaric acid, an ion channel regulator, regulates calcium and oxidative phosphorylation pathways to inhibit breast cancer proliferation and metastasis

Bitem. _ancer i: the globally most conumn malignant mami and the biggest threat to women. ven thou;sh the diagnosis and treatment C, treast cancer are progressing commually, a large number of breast cancer patients eventually tevelop a metastatic tumor, especially tiple-negative breast cancer (TNBC). Recently, metal ion homeostasis and ion signaling pathway have becorne imponant targets for cancer therapy In this stttdy, We analyzed the ef iota ects and rnechanisms of isopimaric acid (IPA), an ion channel regttlator, on the proliferation and metastasis of breast cancer cells (4 T1, MDA- M B-231and MCF-7) by celi functional assay, Flow cytometry, westem blot, proteornics and other techniqttes in vitro and in vivo. Results found that IPA significantly inhibited the proliferation and metastasis of breast cancer cells (especially 4 T1). Furthei studies on the anti-tumor mechanism of IPA suggested that ,PA might affect EMT and Wnt signaling pathways by targeting mitochondria oxidative phosphorylation and Ca2 signaling pathways, and then inducing breast cancer cef cycle anest and apoptosis. Our research reveals the therapeutic valite of IPA in breast cancer and provides a theoretical basis for the new treatment of breast cancer.