Untargeted metagenomics protocol for the diagnosis of infection from CSF and tissue from sterile sites

被引：6

作者：

Atkinson, Laura ^{[1
]}

Lee, Jack CD. ^{[1
]}

Lennon, Alexander ^{[1
]}

Shah, Divya ^{[1
]}

Storey, Nathaniel ^{[1
]}

Morfopoulou, Sofia ^{[1
,2
]}

Harris, Kathryn A. ^{[3
]}

Breuer, Judy ^{[1
,2
]}

Brown, Julianne R. ^{[1
]}

机构：

[1] Great Ormond St Hosp Children NHS Fdn Trust, Dept Microbiol Virol & Infect Control, London, England

[2] UCL, Inst Child Hlth, Dept Infect Immun & Inflammat, London, England

[3] Barts Hlth NHS Trust, Royal London Hosp, Dept Virol, London, England

来源：

HELIYON | 2023年 / 9卷 / 09期

关键词：

ASTROVIRUS VA1/HMO-C;

D O I：

10.1016/j.heliyon.2023.e19854

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Metagenomic next-generation sequencing (mNGS) is an untargeted technique capable of detecting all microbial nucleic acid within a sample. This protocol outlines our wet laboratory method for mNGS of cerebrospinal fluid (CSF) specimens and tissues from sterile sites. We use this method routinely in our clinical service, processing 178 specimens over the past 2.5 years in a laboratory that adheres to ISO:15189 standards. We have successfully used this protocol to diagnose multiple cases of encephalitis and hepatitis.

引用

页数：14

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