A Portable Nanozyme-Strip Fabricated by Ru-ZIF-8 with Dual Nanozymatic Activity for Detection of Acetylcholine

被引：4
作者：
Cui, Xin [1 ]
Zhang, Jiachen [1 ]
Liu, Yitong [1 ]
Shao, Wen [1 ]
Tang, Yawen [1 ]
Sun, Hanjun [1 ]
机构：
[1] Nanjing Normal Univ, Jiangsu Collaborat Innovat Ctr Biomed Funct Mat, Sch Chem & Mat Sci, Jiangsu Key Lab New Power Batteries, Nanjing 210023, Peoples R China
来源：
CHEMNANOMAT | 2023年 / 9卷 / 10期
基金：
中国国家自然科学基金;
关键词：
esterase-like activity; peroxidase-like activity; nanozyme with dual nanozymatic activities; detection acetylcholine; nanozyme-strip; PEROXIDASE-LIKE ACTIVITY; CHOLINE; NANOPARTICLES; BIOSENSORS; GLUCOSE; ENZYMES; SYSTEM;
D O I：
10.1002/cnma.202300310
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
In the present study, the incorporation of Ru into the framework of ZIF-8 resulted in that a Ru-ZIF-8 nanozyme with both esterase-like activity and peroxidase-like activity. Owing to the dual nanozymatic activities of Ru-ZIF-8 nanozyme, the acetylcholine (ACh) detection process utilized only one natural enzyme (choline oxidase), which greatly simplified the detection procedure. In the detection process, nanozyme Ru-ZIF-8 showed esterase-like activity to catalyze the hydrolysis of ACh to choline. Then the generated choline was oxidized by choline oxidase to generate H2O2. Finally, Ru-ZIF-8 with peroxidase-like activity was used to catalyze H2O2 to oxidize 2,2 & PRIME;-azino-bis (3-ethylbenzothiazo-line-6-sulfonic acid) ammonium salt with a color change. As a result, the linear range for ACh was from 0.01 & mu;M-22 & mu;M with the detection limit (DL) of 5.5 nM. In addition, Ru-ZIF-8 was utilized to fabricate a portable detection test strip. Our work provided a simple, portable, low-cost, highly sensitive and selective assay for the detection of ACh, which paved the way for the development of multi enzyme cascade assays.
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页数：7
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