Loop-mediated isothermal amplification assay in combination with lateral flow dipstick in HSV type 1

被引：0
|
作者：
Gao, Fei [1 ,3 ]
Wang, Zhuoshi [2 ]
Zhang, Mingqi [2 ]
Zhao, Yan [2 ]
Yao, Tao [1 ]
Xu, Ling [1 ]
He, Xiangdong [2 ]
Wang, Bing [3 ]
He, Wei [1 ,2 ]
机构：
[1] He Univ, HE Eye Hosp, Clin Res Ctr, Shenyang, Liaoning, Peoples R China
[2] He Univ, Precis Med Innovat Inst, Stem Cell Res Ctr, Shenyang, Liaoning, Peoples R China
[3] Northeastern Univ, Coll Life Sci & Hlth, Shenyang, Liaoning, Peoples R China
来源：
FUTURE VIROLOGY | 2023年 / 18卷 / 01期
关键词：
HSV type 1; herpetic keratitis; lateral flow dipstick; loop-mediated isothermal amplification; quantitative PCR; HERPES-SIMPLEX-VIRUS; DIAGNOSIS; KERATITIS; LAMP;
D O I：
10.2217/fvl-2022-0100
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Aim: Diagnostic methods for HSV-1 need to be updated. Materials & methods: Serial dilutions of HSV-1 DNA and ocular pathogenic microorganisms were tested by loop-mediated isothermal amplification-lateral flow dipstick assay (LAMP-LFD assay). The reliability of the LAMP-LFD assay and quantitative real-time PCR (qPCR) for HSV-1 detection was also validated using 30 clinical swab samples. Results: The LAMP reaction is optimal at 65 degrees C and 30 min. And the LAMP-LFD assay could detect HSV-1 up to 10 copies/mu l and no cross-reactivity with other pathogens was observed. Moreover, LAMP-LFD had similar positive rates to qPCR in clinical samples, and sensitivity and specificity were highly consistent with those of qPCR. Conclusion: LAMP-LFD assay is a rapid technique for the diagnosis of HSV-1.
引用
收藏
页码：39 / 50
页数：12
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