Virulent potential of methicillin-resistant and methicillin-susceptible Staphylococcus pseudintermedius in dogs

被引：9

作者：

Breyer, Gabriela Merker ^{[1
,2
]}

Saggin, Bianca Fagundes ^{[1
,2
]}

de Carli, Silvia ^{[1
,2
]}

da Silva, Maria Eduarda Rocha Jacques ^{[1
,2
]}

da Costa, Mateus Matiuzzi ^{[3
]}

Brenig, Bertram ^{[4
]}

Azevedo, Vasco Ariston de Carvalho ^{[5
]}

Cardoso, Marisa Ribeiro de Itapema ^{[2
,6
]}

Siqueira, Franciele Maboni ^{[1
,2
]}

机构：

[1] Univ Fed Rio Grande Sul UFRGS, Lab Bacteriol Vet LaBacVet, Dept Patol Vet, Porto Alegre, Brazil

[2] Univ Fed Rio Grande Sul UFRGS, Programa Posgrad Ciencias Vet, Porto Alegre, Brazil

[3] Univ Fed Vale Sao Francisco UNIVASF, Dept Zootecnia, Petrolina, Brazil

[4] Georg August Univ Gottingen, Inst Vet Med, Dept Mol Biol Livestock, D-37077 Gottingen, Germany

[5] Univ Fed Minas Gerais UFMG, Inst Ciencias Biol, Lab Genet Mol & Celular LGCM, Belo Horizonte, Brazil

[6] Univ Fed Rio Grande Sul UFRGS, Lab Med Vet Prevent, Dept Patol Vet, Porto Alegre, Brazil

来源：

ACTA TROPICA | 2023年 / 242卷

关键词：

MRSP; MSSP; Biofilm formation; Virulence factors; Antibiotic resistance; Multidrug resistance; Canine infection; SYNDROME TOXIN 1; EXFOLIATIVE TOXIN; ANTIMICROBIAL RESISTANCE; BIOFILM-FORMATION; RAPID EMERGENCE; MEC SCCMEC; INTERMEDIUS; PREVALENCE; HEALTHY; AUREUS;

D O I：

10.1016/j.actatropica.2023.106911

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Staphylococcus pseudintermedius is a zoonotic pathogen responsible for several infectious diseases in pet animals, yet its pathogenic potential is not fully understood. Thus, this study aims to unravel the virulence profile of S. pseudintermedius from canine origin. Methicillin-resistant (MRSP) and methicillin-susceptible (MSSP) strains were isolated from different infection sites and their genotypic and phenotypic features were compared to determine the clinical implications of MRSP and MSSP strains. Bacterial identification was performed using MALDI-TOF and 16S-rDNA sequencing. In addition, we used multilocus sequence typing (MLST) for strains' sequence type (ST) determination and phylogenetic relationship. The strains were screened for toxin genes, including cytotoxins (lukS, lukF), exfoliative toxin (siet), enterotoxins (sea, seb, sec, secCanine, sel, sem, and seq) and toxic shock syndrome toxin (tst-1). In vitro phenotypic analyses assessing antimicrobial susceptibility profile, biofilm formation ability, and expression of extracellular matrix components were performed. The investigated S. pseudintermedius strains belong to 17 unique ST, most of which were classified as ST71. MSSP and MRSP strains shared siet, lukS, and lukF virulence markers. Our findings showed that some MSSP strains also harbored sel, seq, and sem enterotoxin genes, suggesting a more diverse virulence profile. All MRSP strains and 77% of MSSP strains were classified as multidrug resistant (MDR). Moreover, all investigated S. pseudintermedius strains showed strong biofilm formation ability. In summary, our findings highlight the wide spread of highly virulent and drug-resistant zoonotic S. pseudintermedius strains, being a potential concern for One Health issues.

引用

页数：9

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