Ribosomal proteins regulate 2-cell-stage transcriptome in mouse embryonic stem cells

被引:8
|
作者
Yi, Yao [1 ,2 ]
Zeng, Yingying [1 ,3 ]
Sam, Tsz Wing [1 ,2 ,10 ]
Hamashima, Kiyofumi [1 ,2 ]
Tan, Rachel Jun Rou [1 ]
Warrier, Tushar [1 ]
Phua, Jun Xiang [1 ]
Taneja, Reshma [10 ]
Liou, Yih-Cherng [2 ]
Li, Hu [4 ]
Xu, Jian [5 ,6 ,7 ]
Loh, Yuin-Han [1 ,2 ,8 ,9 ,11 ]
机构
[1] ASTAR, Inst Mol & Cell Biol, Div Cell Biol & Therapies, Cell Fate Engn & Therapeut Lab, Singapore 138673, Singapore
[2] Natl Univ Singapore, Dept Biol Sci, Singapore 117558, Singapore
[3] Nanyang Technol Univ, Sch Biol Sci, Singapore 637551, Singapore
[4] Mayo Clin, Ctr Individualized Med, Dept Mol Pharmacol & Expt Therapeut, Rochester, MN 55905 USA
[5] Radboud Univ Nijmegen, Inst Water & Wetland Res, Dept Plant Syst Physiol, Heyendaalseweg 135, NL-6525 AJ Nijmegen, Netherlands
[6] Natl Univ Singapore, Ctr BioImaging Sci, Dept Biol Sci, Singapore 117543, Singapore
[7] Shandong Agr Univ, Joint Ctr Single Cell Biol, Tai An 271018, Shandong, Peoples R China
[8] Natl Univ Singapore, NUS Grad Sch Integrat Sci, Singapore 119077, Singapore
[9] Natl Univ Singapore, Engn Programme, Singapore 119077, Singapore
[10] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Physiol, Hlth Longev Translat Res Program, Singapore 117593, Singapore
[11] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Physiol, Singapore 117593, Singapore
来源
STEM CELL REPORTS | 2023年 / 18卷 / 02期
基金
美国国家卫生研究院;
关键词
C-MYC; ACTIVATION; P53; DNA; PLURIPOTENCY; EXPRESSION; GROWTH; L11; PROLIFERATION; CHROMATIN;
D O I
10.1016/j.stemcr.2022.12.007
中图分类号
Q813 [细胞工程];
学科分类号
摘要
A rare sub-population of mouse embryonic stem cells (mESCs), the 2-cell-like cell, is defined by the expression of MERVL and 2-cell-stagespecific transcript (2C transcript). Here, we report that the ribosomal proteins (RPs) RPL14, RPL18, and RPL23 maintain the identity of mESCs and regulate the expression of 2C transcripts. Disregulation of the RPs induces DUX-dependent expression of 2C transcripts and alters the chromatin landscape. Mechanically, knockdown (KD) of RPs triggers the binding of RPL11 to MDM2, an interaction known to prevent P53 protein degradation. Increased P53 protein upon RP KD further activates its downstream pathways, including DUX. Our study delineates the critical roles of RPs in 2C transcript activation, ascribing a novel function to these essential proteins.
引用
收藏
页码:463 / 474
页数:12
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