The Expression Patterns and Implications of MALAT1, MANCR, PSMA3-AS1 and miR-101 in Esophageal Adenocarcinoma

被引:0
作者
Syllaios, Athanasios [1 ]
Gazouli, Maria [2 ]
Vailas, Michail [1 ]
Mylonas, Konstantinos S. [3 ]
Sakellariou, Stratigoula [4 ]
Sougioultzis, Stavros [5 ]
Karavokyros, Ioannis [1 ]
Liakakos, Theodoros [1 ]
Schizas, Dimitrios [1 ]
机构
[1] Natl & Kapodistrian Univ Athens, Laikon Gen Hosp, Dept Surg 1, Athens 11527, Greece
[2] Natl & Kapodistrian Univ Athens, Med Sch, Dept Basic Med Sci, Lab Biol, Athens 11527, Greece
[3] Onassis Cardiac Surg Ctr, Dept Cardiac Surg, Athens 17671, Greece
[4] Natl & Kapodistrian Univ Athens, Med Sch, Dept Pathol 1, Athens 11527, Greece
[5] Natl & Kapodistrian Univ Athens, Sch Med, Dept Pathophysiol, Gastroenterol Unit, Athens 11527, Greece
关键词
LncRNA; miRNA; MALAT1; MANCR; PSMA3-AS1; miR-101; esophageal cancer; adenocarcinoma; LONG NONCODING RNAS; PROLIFERATION; APOPTOSIS; MIGRATION; INVASION; CELLS;
D O I
10.3390/ijms25010098
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Esophageal adenocarcinoma (EAC) is a malignant tumor with poorly understood molecular mechanisms. This study endeavors to elucidate how the long non-coding RNAs (lncRNAs) MALAT1, MANCR and PSMA3-AS1, as well as the microRNA miR-101, exhibit specific expression patterns in the pathogenesis and prognosis of EAC. A total of 50 EAC tissue samples (tumors and lymph nodes) and a control group comprising 26 healthy individuals were recruited. The samples underwent quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analyses. The relative expression levels of MALAT1, MANCR, PSMA3-AS1, and miR-101 were ascertained and correlated with various clinicopathological parameters including TNM staging, tumor characteristics (size and grade of the tumor) lymphatic invasion, disease-free (DFS) and overall survival (OS) of EAC patients. Quantitative analyses revealed that MALAT1 and MANCR were significantly upregulated in EAC tumors and positive lymph nodes when compared to control tissues (p < 0.05). Such dysregulations correlated positively with advanced lymphatic metastases and a higher N stage. DFS in the subgroup of patients with negative lymph nodes was higher in the setting of low-MANCR-expression patients compared to patients with high MANCR expression (p = 0.02). Conversely, miR-101 displayed a significant downregulation in EAC tumors and positive lymph nodes (p < 0.05), and correlated negatively with advanced tumor stage, lymphatic invasion and the grade of the tumor (p = 0.006). Also, patients with low miR-101 expression showed a tendency towards inferior overall survival. PSMA3-AS1 did not demonstrate statistically significant alterations (p > 0.05). This study reveals MALAT1, MANCR, and miR-101 as putative molecular markers for prognostic evaluation in EAC and suggests their involvement in EAC progression.
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页数:14
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共 48 条
[1]  
Dong XQ, 2019, EUR REV MED PHARMACO, V23, P5187, DOI 10.26355/eurrev_201906_18183
[2]   RNA-RNA Interactions Enable Specific Targeting of Noncoding RNAs to Nascent Pre-mRNAs and Chromatin Sites [J].
Engreitz, Jesse M. ;
Sirokman, Klara ;
McDonel, Patrick ;
Shishkin, Alexander A. ;
Surka, Christine ;
Russell, Pamela ;
Grossman, Sharon R. ;
Chow, Amy Y. ;
Guttman, Mitchell ;
Lander, Eric S. .
CELL, 2014, 159 (01) :188-199
[3]  
Fan Jingxian, 2021, J BUON, V26, P1517
[4]   Keeping abreast with long non-coding RNAs in mammary gland development and breast cancer [J].
Hansji, Herah ;
Leung, Euphemia Y. ;
Baguley, Bruce C. ;
Finlay, Graeme J. ;
Askarian-Amiri, Marjan E. .
FRONTIERS IN GENETICS, 2014, 5
[5]   LncRNA-MALAT1: A Key Participant in the Occurrence and Development of Cancer [J].
Hao, Longhui ;
Wu, Wenzheng ;
Xu, Yankun ;
Chen, Yufan ;
Meng, Chengzhen ;
Yun, Jingyi ;
Wang, Xiaoyu .
MOLECULES, 2023, 28 (05)
[6]   Downregulation of miR-101 in gastric cancer correlates with cyclooxygenase-2 overexpression and tumor growth [J].
He, Xiao-Pu ;
Shao, Yun ;
Li, Xiao-Lin ;
Xu, Wei ;
Chen, Guo-Sheng ;
Sun, Huan-Huan ;
Xu, Hai-Chen ;
Xu, Xian ;
Tang, Dan ;
Zheng, Xi-Feng ;
Xue, Yi-Ping ;
Huang, Guo-Chang ;
Sun, Wei-Hao .
FEBS JOURNAL, 2012, 279 (22) :4201-4212
[7]   Mitotically associated long non-coding RNA is a tumor promoter in anaplastic thyroid cancer [J].
Huang, Nai-Si ;
Lei, Bo-Wen ;
Tan, Li-Cheng ;
Yu, Peng-Cheng ;
Shi, Xiao ;
Wang, Yu ;
Ji, Qing-Hai ;
Wei, Wen-Jun ;
Lu, Zhong-Wu ;
Wang, Yu-Long .
ANNALS OF TRANSLATIONAL MEDICINE, 2020, 8 (19)
[8]   MicroRNA-98 and microRNA-214 post-transcriptionally regulate enhancer of zeste homolog 2 and inhibit migration and invasion in human esophageal squamous cell carcinoma [J].
Huang, Sheng-Dong ;
Yuan, Yang ;
Zhuang, Chong-Wen ;
Li, Bai-Ling ;
Gong, De-Jun ;
Wang, Shu-Gang ;
Zeng, Zhi-Yong ;
Cheng, He-Zhong .
MOLECULAR CANCER, 2012, 11
[9]   Long non-coding RNA PSMA3-AS1 promotes glioma progression through modulating the miR-411-3p/HOXA10 pathway [J].
Huang, Tianzao ;
Chen, Yingxian ;
Zeng, Yile ;
Xu, Chaoyang ;
Huang, Jinzhong ;
Hu, Weipeng ;
Chen, Xiangrong ;
Fu, Huangde .
BMC CANCER, 2021, 21 (01)
[10]   Low plasma levels of miR-101 are associated with tumor progression in gastric cancer [J].
Imamura, Taisuke ;
Komatsu, Shuhei ;
Ichikawa, Daisuke ;
Miyamae, Mahito ;
Okajima, Wataru ;
Ohashi, Takuma ;
Kiuchi, Jun ;
Nishibeppu, Keiji ;
Kosuga, Toshiyuki ;
Konishi, Hirotaka ;
Shiozaki, Atsushi ;
Okamoto, Kazuma ;
Fujiwara, Hitoshi ;
Otsuji, Eigo .
ONCOTARGET, 2017, 8 (63) :106538-106550