The CAP superfamily protein PsCAP1 secreted by Phytophthora triggers immune responses in Nicotiana benthamiana through a leucine-rich repeat receptor-like protein

被引：7

作者：

Jiang, Haibin ^{[1
,2
,3
]}

Xia, Yeqiang ^{[1
,2
,3
]}

Zhang, Sicong ^{[1
,2
,3
]}

Zhang, Zhichao ^{[1
,2
,3
]}

Feng, Hui ^{[1
,2
,3
]}

Zhang, Qi ^{[1
,2
,3
]}

Chen, Xi ^{[1
,2
,3
]}

Xiao, Junhua ^{[1
,2
,3
]}

Yang, Sen ^{[1
,2
,3
]}

Zeng, Mengzhu ^{[1
,2
,3
]}

Chen, Zhaodan ^{[1
,2
,3
]}

Ouyang, Haibing ^{[1
,2
,3
]}

He, Xinyi ^{[1
,2
,3
]}

Sun, Guangzheng ^{[1
,2
,3
]}

Wu, Jinbin ^{[1
,2
,3
]}

Dong, Suomeng ^{[1
,2
,3
]}

Ye, Wenwu ^{[1
,2
,3
]}

Ma, Zhenchuan ^{[1
,2
,3
]}

Wang, Yan ^{[1
,2
,3
]}

Wang, Yuanchao ^{[1
,2
,3
]}

机构：

[1] Nanjing Agr Univ, Dept Plant Pathol, Nanjing 210095, Jiangsu, Peoples R China

[2] Nanjing Agr Univ, Key Lab Plant Immun, Nanjing 210095, Jiangsu, Peoples R China

[3] Nanjing Agr Univ, Key Lab Soybean Dis & Pest Control, Minist Agr & Rural Affairs, Nanjing 210095, Peoples R China

来源：

NEW PHYTOLOGIST | 2023年 / 240卷 / 02期

基金：

中国国家自然科学基金;

关键词：

apoplastic effector; CAP superfamily protein; LRR-RLP; Phytophthora; plant immunity; PLANT; EFFECTOR; TOMATO; RECOGNITION; PERCEPTION; ACTIVATION; KINASE; DOMAIN; PREKALLIKREIN; RESISTANCE;

D O I：

10.1111/nph.19194

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

center dot The role of cysteine-rich secretory proteins, antigen 5, and pathogenesis-related 1 (CAP) superfamily proteins in the innate immune responses of mammals is well characterized. However, the biological function of CAP superfamily proteins in plant-microbe interactions is poorly understood. center dot We used proteomics and transcriptome analyses to dissect the apoplastic effectors secreted by the oomycete Phytophthora sojae during early infection of soybean leaves. By transiently expressing these effectors in Nicotiana benthamiana, we identified PsCAP1, a novel type of secreted CAP protein that triggers immune responses in multiple solanaceous plants including N. benthamiana. This secreted CAP protein is conserved among oomycetes, and multiple PsCAP1 homologs can be recognized by N. benthamiana. center dot PsCAP1-triggered immune responses depend on the N-terminal immunogenic fragment (aa 27-151). Pretreatment of N. benthamiana with PsCAP1 or the immunogenic fragment increases plant resistance against Phytophthora. The recognition of PsCAP1 and different homologs requires the leucine-rich repeat receptor-like protein RCAP1, which associates with two central receptor-like kinases BRI1-associated receptor kinase 1 (BAK1) and suppressor of BIR1-1 (SOBIR1) in planta. center dot These findings suggest that the CAP-type apoplastic effectors act as an important player in plant-microbe interactions that can be perceived by plant membrane-localized receptor to activate plant resistance.

引用

页码：784 / 801

页数：18

共 60 条

[21] FLS2:: An LRR receptor-like kinase involved in the perception of the bacterial elicitor flagellin in Arabidopsis [J].