Optimization of in situ cellulase production from Penicillium oxalicum P-07 under submerged fermentation conditions with different cellulose types

Biological enzymes have attracted much attention due to their good performance and environmentally friendly characteristics. This case focused on promoting the production of cellulase from a fungus strain (identified as Penicillium oxalicum) was isolated from food waste (FW). To better apply Penicillium oxalicum P-07 to cellulose degradation, the optimization of process parameters is particularly important. The combination of medium substrates sodium carboxymethyl cellulose (CMC-Na) and wheat bran (WB) presented higher filter paper activity (FPase) than the sum of the sole ones. Further optimizing the degradation conditions to pH 5.0 and 37 celcius, the FPase increased by 1.5 times, from 0.30 U/mL to 0.45 U/mL. Meanwhile, endoglucanase (CMCase), cellobiohydrolases (CBHase) and 8-glucosidase reached 5.66, 0.25 and 5.59 U/mL respectively. The outcomes demonstrated that Penicillium oxalicum P-07 cellulase production was greatly aided by the optimization of operation parameters.