Two-Phase Fermentation Systems for Microbial Production of Plant-Derived Terpenes

被引：13

作者：

Li, Tuo ^{[1
]}

Liu, Ximeng ^{[1
]}

Xiang, Haoyu ^{[1
]}

Zhu, Hehua ^{[1
]}

Lu, Xuan ^{[1
]}

Feng, Baomin ^{[1
]}

机构：

[1] Dalian Univ, Coll Life & Hlth, Dalian 116622, Peoples R China

来源：

MOLECULES | 2024年 / 29卷 / 05期

关键词：

two-phase fermentation; plant-derived terpenes; microbial cell factory; in situ extraction; biosynthesis; downstream processing; HIGH-LEVEL PRODUCTION; RECOMBINANT ESCHERICHIA-COLI; SACCHAROMYCES-CEREVISIAE; MEVALONATE PATHWAY; BIOSYNTHETIC-PATHWAY; YARROWIA-LIPOLYTICA; HETEROLOGOUS BIOSYNTHESIS; HYDROCARBON DEGRADATION; DIPHOSPHATE SYNTHASE; LINALOOL PRODUCTION;

D O I：

10.3390/molecules29051127

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Microbial cell factories, renowned for their economic and environmental benefits, have emerged as a key trend in academic and industrial areas, particularly in the fermentation of natural compounds. Among these, plant-derived terpenes stand out as a significant class of bioactive natural products. The large-scale production of such terpenes, exemplified by artemisinic acid-a crucial precursor to artemisinin-is now feasible through microbial cell factories. In the fermentation of terpenes, two-phase fermentation technology has been widely applied due to its unique advantages. It facilitates in situ product extraction or adsorption, effectively mitigating the detrimental impact of product accumulation on microbial cells, thereby significantly bolstering the efficiency of microbial production of plant-derived terpenes. This paper reviews the latest developments in two-phase fermentation system applications, focusing on microbial fermentation of plant-derived terpenes. It also discusses the mechanisms influencing microbial biosynthesis of terpenes. Moreover, we introduce some new two-phase fermentation techniques, currently unexplored in terpene fermentation, with the aim of providing more thoughts and explorations on the future applications of two-phase fermentation technology. Lastly, we discuss several challenges in the industrial application of two-phase fermentation systems, especially in downstream processing.

引用

页数：37

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