Paper based colorimetric sensor using novel green magnetized nanocomposite of pinus for hydrogen peroxide detection in water and milk

The present work was focused on the Hydrogen peroxide (H2O2) detection by designing a portable and sustainable filter paper based sensor based on novel use of green enzyme mimic magnetic nanoparticles of Pinus needles (MNP). The nanozymes were synthesized via an easy ecofriendly and cheap route besides characterized by UV-Vis spectroscopy, Scanning Electron Microscope, Fourier transform infrared spectroscopy, Zeta potential, X-ray diffraction and Vibrating Sample Magnetometer etc. The characterization study supported the suitability and optical properties of MNP for sensor fabrication like presence of various functional groups, availability of binding sites, moderate stability, super paramagnetic properties and amorphous structure etc. The coactive effect of Pinus needles and magnetic nanoparticles components on sensing platform demonstrated an inherent peroxidase-like activity with enhanced optical response for catalyzing the oxidation of substrate 3,3 & PRIME;,5,5 & PRIME;-tetra-methylbenzidine with perceived naked eye reaction. The optimization was carried out with respect to different parameters such as incubation time (15 min), Gel concentration (0.2 mg/mL) and concentration of MNP (2 mg/ mL) etc. A good sensitivity was achieved by using color analyzing model at a lower limit of detection (LOD) 0.007 & mu;M in a relative wide-ranging of 0.01 & mu;M-0.09 & mu;M. The application in biological and samples environmental (tap water and milk) indicated that the proposed paper-based sensor delivers reasonable platforms for simple, sustainable, and quick detection of H2O2 with high selectivity.