Dwell Time Prolongation and Identification of Single Nucleotides Passing through a Solid-State Nanopore by Using Ammonium Sulfate Aqueous Solution

被引：1

作者：

Yanagi, Itaru ^{[1
]}

Akahori, Rena ^{[2
]}

Takeda, Ken-ichi ^{[2
]}

机构：

[1] Hitachi Ltd, Ctr Exploratory Res, Res & Dev Grp, Kokubunji, Tokyo 1858603, Japan

[2] Hitachi Ltd, Ctr Technol Innovat Healthcare, Res & Dev Grp, Kokubunji, Tokyo 1858603, Japan

来源：

ACS OMEGA | 2023年 / 8卷 / 23期

关键词：

DNA TRANSLOCATION; STRANDED-DNA; GENOME; NOISE; SLOW; MESH;

D O I：

10.1021/acsomega.3c02703

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The ionic current blockades when poly-(dT)(60) or dNTPspassed through SiN nanopores in an aqueous solution containing (NH4)(2)SO4 were investigated. The dwell timeof poly-(dT)(60) in the nanopores in an aqueous solution containing(NH4)(2)SO4 was significantly longercompared to that in an aqueous solution that did not contain (NH4)(2)SO4. This dwell time prolongationeffect due to the aqueous solution containing (NH4)(2)SO4 was also confirmed when dCTP passed throughthe nanopores. In addition, when the nanopores were fabricated viadielectric breakdown in the aqueous solution containing (NH4)(2)SO4, the dwell time prolongation effect fordCTP still occurred even after the aqueous solution was displacedwith the aqueous solution without (NH4)(2)SO4. Furthermore, we measured the ionic current blockades whenthe four types of dNTPs passed through the same nanopore, and thefour types of dNTPs could be statistically identified according totheir current blockade values.

引用

页码：21285 / 21292

页数：8