lncRNA HCG22 regulated cell growth and metastasis of papillary thyroid cancer via negatively modulating miR-425-5p

被引：5

作者：

Cao, Xuepeng ^{[1
]}

Ma, Chuang ^{[2
]}

Wu, Yang ^{[3
]}

Huang, Jianyuan ^{[3
]}

机构：

[1] Hexi Univ, Med Sch, Zhangye, Peoples R China

[2] Beijing Haidian Hosp, Surg Wound Dept, Beijing, Peoples R China

[3] First Peoples Hosp Neijiang, Dept Gen Surg Thyroid Gland Blood Vessel, 1866 West Sect Hanan Ave, Neijiang 641099, Peoples R China

来源：

ENDOKRYNOLOGIA POLSKA | 2024年 / 75卷 / 01期

关键词：

papillary thyroid cancer; cell growth; migration; invasion; ceRNA; miR-425-5p; IDENTIFICATION; CERNA;

D O I：

10.5603/ep.97425

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Introduction: Papillary thyroid cancer (PTC) is a common malignant tumour in the endocrine system with increasing incidence. LncRNA HCG22 (HCG22) was noticed to be dysregulated in PTC, but its specific function and mechanism remain unknown. The function of HCG22 and its underlying molecular mechanism was investigated to evaluate its potential as a biomarker for PTC. Material and metbods: The expression of HCG22 was detected in PTC cells (TPC-1, SNU790, GLAG-66, and BCPAP) and normal thyroid cells (Nthy-ori) using real time quantative polymerase chain reaction (RT-qPCR). HCG22 and miR-425-5p were regulated by cell transfection. The cell proliferation and metastasis were assessed by CCK8 and Transwell assay. Results: HCG22 was upregulated in PTC cells, of which the knockdown suppressed the proliferation, migration, and invasion of PTC cells. miR-425-5p was downregulated in PTC cells, which was negatively regulated by HCG22. Silencing miR-425-5p could reverse the inhibitory effect of HCG22 knockdown on the cellular processes of PTC. Conclusions: HCG22 served as a tumour promoter in PTC cells, which regulated cell proliferation and metastasis via negatively regulating miR-425-5p.

引用

页码：20 / 26

页数：7

共 32 条

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