Dietary choline, but not L-carnitine, increases circulating lipid and lipoprotein concentrations, without affecting body composition, energy expenditure or respiratory quotient in lean and obese male cats during weight maintenance

被引：1

作者：

Rankovic, Alexandra ^{[1
]}

Verton-Shaw, Shoshana ^{[2
]}

Shoveller, Anna K. K. ^{[3
]}

Bakovic, Marica ^{[4
]}

Kirby, Gordon ^{[1
]}

Verbrugghe, Adronie ^{[2
]}

机构：

[1] Univ Guelph, Ontario Vet Coll, Dept Biomed Sci, Guelph, ON, Canada

[2] Univ Guelph, Ontario Vet Coll, Dept Clin Studies, Guelph, ON, Canada

[3] Univ Guelph, Ontario Agr Coll, Dept Anim Biosci, Guelph, ON, Canada

[4] Univ Guelph, Coll Biol Sci, Dept Human Hlth & Nutr Sci, Guelph, ON, Canada

来源：

FRONTIERS IN VETERINARY SCIENCE | 2023年 / 10卷

基金：

加拿大自然科学与工程研究理事会;

关键词：

dual-energy x-ray absorptiometry; feline nutrition; indirect calorimetry; methionine; methyl donor; one carbon metabolism; pet obesity; HEPATIC LIPIDOSIS; ALKALINE-PHOSPHATASE; INSULIN SENSITIVITY; GLUCOSE-TOLERANCE; CONDITION SCORE; DOMESTIC CAT; PLASMA; SUPPLEMENTATION; PROFILE; LIVER;

D O I：

10.3389/fvets.2023.1198175

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

IntroductionDue to the involvement in one-carbon metabolism and lipid mobilization, choline and L-carnitine supplementation have been recommended to minimize hepatic lipid accumulation and support fat oxidation, respectively. This study investigated the lipotropic benefits of choline or L-carnitine supplementation in lean and obese cats maintaining body weight (BW). MethodsLean [n = 9; body condition score (BCS): 4-5/9] and obese (n = 9; BCS: 8-9/9) adult male neutered colony cats were used in a replicated 3 x 3 complete Latin square design. Treatments included choline (378 mg/kg BW0.67), L-carnitine (200 mg/kg BW) and control (no supplement). Treatments were supplemented to the food for 6 weeks each, with a 2-week washout between treatments. Cats were fed once daily to maintenance energy requirements, and BW and BCS were assessed weekly. Fasted blood collection, indirect calorimetry, and dual-energy X-ray absorptiometry occurred at the end of each treatment period. Serum was analyzed for cholesterol (CHOL), high-density lipoprotein CHOL (HDL-C), triglycerides (TAG), non-esterified fatty acids (NEFA), glucose, creatinine (CREAT), urea, alkaline phosphatase (ALP) and alanine aminotransferase (ALT). Very low-density lipoprotein CHOL (VLDL) and low-density lipoprotein CHOL (LDL-C) were calculated. Data were analyzed using proc GLIMMIX, with group and period as random effects, and treatment, body condition, and their interaction as fixed effects, followed by a Tukey's post-hoc test when significance occurred. ResultsCats supplemented choline had lower food intake (P = 0.025). Treatment did not change BW, BCS and body composition (P > 0.05). Obese cats had greater ALP, TAG, and VLDL, and lower HDL-C compared to lean cats (P < 0.05). Choline resulted in greater CHOL, HDL-C, LDL-C and ALT (P < 0.05). L-carnitine resulted in lower CREAT (P = 0.010). Following the post-hoc test, differences between treatment means were not present for ALP (P = 0.042). No differences were found for glucose, urea or NEFA (P > 0.05). Obese cats had a lower fed respiratory quotient (RQ), regardless of treatment (P = 0.045). Treatment did not affect fed or fasted RQ and energy expenditure (P > 0.05). DiscussionCholine appeared to increase circulating lipid and lipoprotein concentrations regardless of body condition, likely through enhanced lipid mobilization and hepatic elimination. Neither dietary choline or L-carnitine altered body composition or energy metabolism in the lean or obese cats, as compared to control.

引用

页数：14