Antimicrobial Resistance and Extended-Spectrum Beta-Lactamase Genes in Enterobacterales, Pseudomonas and Acinetobacter Isolates from the Uterus of Healthy Mares

被引:3
作者
Thomson, Pamela [1 ]
Garcia, Patricia [2 ]
del Rio, Camila [1 ]
Castro, Rodrigo [3 ]
Nunez, Andrea [4 ,5 ]
Miranda, Carolina [6 ]
机构
[1] Univ Andres Bello, Escuela Med Vet, Fac Ciencias Vida, Lab Microbiol Clin & Microbioma, Santiago 8370134, Chile
[2] Pontificia Univ Catolica, Escuela Med, Dept Labs Clin, Santiago 8940000, Chile
[3] Univ Santo Tomas, Fac Recursos Nat & Med Vet, Escuela Med Vet, Talca 3473620, Chile
[4] Univ Catolica Maule, Fac Ciencias Agr & Forestales, Escuela Med Vet, Curico 3340000, Chile
[5] Univ las Amer, Fac Med Vet & Agron, Santiago 7500975, Chile
[6] Pontificia Univ Catolica, Lab Microbiol Red Salud UC CHRISTUS, Santiago 8940000, Chile
来源
PATHOGENS | 2023年 / 12卷 / 09期
关键词
uterus; mares; antibiotic resistance; ESBL; ESCHERICHIA-COLI; INFECTION-CONTROL; ENDOMETRITIS; BAUMANNII; HORSES; SUSCEPTIBILITY; PREVALENCE; MICROBIOME; PATHOGENS; BACTERIA;
D O I
10.3390/pathogens12091145
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Antibiotic-resistant bacteria are a growing concern for human and animal health. The objective of this study was to determine the antimicrobial resistance and extended-spectrum beta-lactamase genes in Enterobacterales, Pseudomonas spp. and Acinetobacter spp. isolates from the uterus of healthy mares. For this purpose, 21 mares were swabbed for samples, which were later seeded on blood agar and MacConkey agar. The isolates were identified using MALDI-TOF and the antimicrobial susceptibility test was performed using the Kirby-Bauer technique. To characterize the resistance genes, a polymerase chain reaction (PCR) scheme was performed. Of the isolates identified as Gram-negative, 68.8% were Enterobacterales, represented by E. coli, Enterobacter cloacae, Citrobacter spp., and Klebsiella pneumoniae; 28.1% belonged to the genus Acinetobacter spp.; and 3.1% to Pseudomonas aeruginosa. A 9.3% of the isolates were multidrug-resistant (MDR), presenting resistance to antibiotics from three different classes, while 18.8% presented resistance to two or more classes of different antibiotics. The diversity of three genes that code for ESBL (bla(TEM), bla(CTX-M) and bla(SHV)) was detected in 12.5% of the strains. The most frequent was bla(SHV), while bla(TEM) and bla(CTX-M) were present in Citrobacter spp. and Klebsiella pneumoniae. These results are an alarm call for veterinarians and their environment and suggest taking measures to prevent the spread of these microorganisms.
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页数:12
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