Generation of single-cell and single-nuclei suspensions from embryonic and adult mouse brains

被引：3
作者：
Lee, Dongjin R. [1 ]
Zhang, Yajun [1 ]
Rhodes, Christopher T. [1 ]
Petros, Timothy J. [1 ]
机构：
[1] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Unit Cellular & Mol Neurodev, NIH, Bethesda, MD 20892 USA
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 01期
关键词：
NEURONS;
D O I：
10.1016/j.xpro.2022.101944
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Efficient protocols to generate single-cell and single-nuclei suspensions are critical for the burgeoning field of single-cell/single-nuclei sequencing. Here we describe procedures to generate single-cell and single-nuclei suspensions from embryonic and adult mouse brains. This protocol can be modified for any brain region and/or neural cell type. For complete details on the use and execution of this protocol, please refer to Lee et al. (2022),1 Rhodes et al. (2022),2 Mahadevan et al. (2021),3 Ekins et al. (2020),4 and Wester et al. (2019).5
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页数：23
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