A simplified courtship conditioning protocol to test learning and memory in Drosophila

被引：4

作者：

Gil-Marti, Beatriz ^{[1
,2
]}

Barredo, Celia G. ^{[1
]}

Pina-Flores, Sara ^{[1
]}

Poza-Rodriguez, Adriana ^{[1
]}

Treves, Gaia ^{[1
]}

Rodriguez-Navas, Carmen ^{[1
]}

Camacho, Lucia ^{[1
]}

Perez-Serna, Atenea ^{[1
]}

Jimenez, Inaki ^{[1
]}

Brazales, Laura ^{[1
]}

Fernandez, Javier ^{[1
]}

Martin, Francisco A. ^{[1
]}

机构：

[1] CSIC, Cajal Inst, Av Dr Arce 37, Madrid 28002, Spain

[2] Autonomous Univ Madrid, Dept Biol, Madrid, Spain

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 01期

关键词：

TRACKING; BEHAVIOR;

D O I：

10.1016/j.xpro.2022.101572

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In Drosophila, a male that has previously been sexually rejected reduces its courtship behavior when confronted again with an unreceptive female. This reduced courting time reflects a memory formation process. Here, we describe a simplified protocol to perform the courtship conditioning assay for assessing the reduced courting time, using regular lab equipment and handmade tools. Every step of the procedure, from raising flies and training to testing and quantification of this memory-related behavior, can be implemented in any practice laboratory.

引用

页数：14

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