Investigating the Differential Circulating microRNA Expression in Adolescent Females with Severe Idiopathic Scoliosis: A Proof-of-Concept Observational Clinical Study

被引：3

作者：

Raimondi, Lavinia ^{[1
]}

De Luca, Angela ^{[1
]}

Gallo, Alessia ^{[2
]}

Perna, Fabrizio ^{[3
]}

Cuscino, Nicola ^{[2
]}

Cordaro, Aurora ^{[1
]}

Costa, Viviana ^{[1
]}

Bellavia, Daniele ^{[1
]}

Faldini, Cesare ^{[4
]}

Scilabra, Simone Dario ^{[5
]}

Giavaresi, Gianluca ^{[1
]}

Toscano, Angelo ^{[3
]}

机构：

[1] IRCCS Ist Ortoped Rizzoli, Sci & Tecnol Chirurg, Via Barbiano 1-10, I-40136 Bologna, Italy

[2] IRCCS ISMETT Ist Mediterraneo Trapianti & Terapie, Dipartimento Ric, I-90127 Palermo, Italy

[3] IRCCS Ist Ortoped Rizzoli, Ortopedia Gen, Via Barbiano 1-10, I-40136 Bologna, Italy

[4] IRCCS Ist Ortoped Rizzoli, Clin Ortoped & Traumatol 1, Via Barbiano 1-10, I-40136 Bologna, Italy

[5] Fdn Ri MED, Dipartimento Ric IRCCS ISMETT, Via Ernesto Tricomi 5, I-90145 Palermo, Italy

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2024年 / 25卷 / 01期

关键词：

adolescent idiopathic scoliosis; microRNAs; osteogenesis; BONE-MINERAL DENSITY; HISTONE DEACETYLASE 3; OSTEOBLAST DIFFERENTIATION; DIAGNOSTIC BIOMARKERS; OXIDATIVE STRESS; GENE; EXOSOMES; PLASMA; OSTEOARTHRITIS; PROLIFERATION;

D O I：

10.3390/ijms25010570

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Adolescent Idiopathic Scoliosis (AIS) is the most common form of three-dimensional spinal disorder in adolescents between the ages of 10 and 18 years of age, most commonly diagnosed in young women when severe disease occurs. Patients with AIS are characterized by abnormal skeletal growth and reduced bone mineral density. The etiology of AIS is thought to be multifactorial, involving both environmental and genetic factors, but to date, it is still unknown. Therefore, it is crucial to further investigate the molecular pathogenesis of AIS and to identify biomarkers useful for predicting curve progression. In this perspective, the relative abundance of a panel of microRNAs (miRNAs) was analyzed in the plasma of 20 AIS patients and 10 healthy controls (HC). The data revealed a significant group of circulating miRNAs dysregulated in AIS patients compared to HC. Further bioinformatic analyses evidenced a more restricted expression of some miRNAs exclusively in severe AIS females. These include some members of the miR-30 family, which are considered promising regulators for treating bone diseases. We demonstrated circulating extracellular vesicles (EVs) from severe AIS females contained miR-30 family members and decreased the osteogenic differentiation of mesenchymal stem cells. Proteomic analysis of EVs highlighted the expression of proteins associated with orthopedic disease. This study provides preliminary evidence of a miRNAs signature potentially associated with severe female AIS and suggests the corresponding vesicular component may affect cellular mechanisms crucial in AIS, opening the scenario for in-depth studies on prognostic differences related to gender and grade.

引用

页数：22