Microfluidic bioanalysis based on nanozymes

As a powerful tool, microfluidics has been widely used in bioanalysis due to the advantages of high portability, fewer sample requirements and rapid detection time. To achieve selective and sensitive detection, enzymes are commonly used as biorecognition elements. However, enzymes used in bioanalysis still suffer from high cost and low stability. Therefore, emerging nanozymes (nanomaterials with enzyme-like characteristics) have been developed to replace enzymes to address their shortcomings. The microfluidic devices based on nanozymes have attracted a lot of interest and shown great promise in bioanalysis via a simple, rapid, and portable approach. This review focuses on recent advances in typical nanozymes and nanozymes involved in cascaded reactions used in microfluidic bioanalysis. Additionally, the future challenges and perspectives are discussed for further development. (c) 2022 Elsevier B.V. All rights reserved.