Gas chromatography-mass spectrometry-based metabolomics reveals the potential mechanism of action of saikosaponin D against DSS-induced acute ulcerative colitis

被引：1

作者：

Yan, Yujiao ^{[1
]}

Yin, Shusheng ^{[1
]}

Bian, Xueyan ^{[2
]}

Li, Junhui ^{[3
]}

Wei, Peng ^{[4
]}

Guo, Xi ^{[5
]}

Zhou, Jingjie ^{[6
]}

Yao, Xiajuan ^{[7
]}

Hu, Hong ^{[7
]}

Kong, Deliang ^{[8
]}

Hua, Haibing ^{[6
]}

Li, Yongming ^{[1
]}

机构：

[1] Nanjing Univ Chinese Med, Jiangsu Collaborat Innovat Ctr Chinese Med Resourc, Sch Med & Holist Integrat Med, Nanjing 210023, Peoples R China

[2] Ningbo Univ, Ningbo 315000, Zhejiang, Peoples R China

[3] Tongji Univ, Putuo Peoples Hosp, Dept Nephrol, Shanghai 200060, Peoples R China

[4] Nanjing Univ Chinese Med, Coll Clin Med 1, Nanjing 210023, Peoples R China

[5] China Pharmaceut Univ, Sch Tradit Chinese Pharm, Nanjing 210009, Peoples R China

[6] Nanjing Univ Chinese Med, Affiliated Jiangyin Hosp, Jiangyin 214400, Peoples R China

[7] Nantong Univ, Affiliated Jiangyin Hosp, Nantong 214400, Peoples R China

[8] Naval Med Univ, Changhai Hosp, Dept Nephrol, Shanghai 200433, Peoples R China

来源：

PHYTOCHEMISTRY LETTERS | 2023年 / 57卷

关键词：

Saikosaponin D; Ulcerative colitis; DSS; Metabolomics; Differential metabolites; Metabolic pathways; METABOLISM; ACTIVATION;

D O I：

10.1016/j.phytol.2023.08.005

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Ulcerative colitis (UC) is a part of the cluster of conditions -known as inflammatory bowel disease. It is caused by a plethora of factors that on a long-term basis result in inflammation of the colon and rectum, with accompanied metabolic disorders. In this study, we found that saikosaponin D could ameliorate the symptoms of acute colitis induced by dextran sulfate sodium (DSS) by improving disease activity index, colon length and histological score. Also, gas chromatography-mass spectrometry (GC-MS)-based metabolomics was used to predict the probable mechanism(s) of action of saikosaponin D in the treatment of UC. We found 9 increased metabolites and 16 decreased metabolites, 11 of which were restored by saikosaponin D administration. The related metabolic pathways were involved in & alpha;-linolenic acid and linoleic acid metabolism, fatty acid biosynthesis, valine, leucine and isoleucine biosynthesis, and glycine, serine and threonine metabolism. The underlying mechanisms of action of saikosaponin D elucidated by GC-MS-based metabolomics, could be further validated in future experiments.

引用

页码：133 / 139

页数：7

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