Construction and immunogenicity of a trypsin-independent porcine epidemic diarrhea virus variant

被引:6
作者
Li, Mingxiang [1 ,2 ]
Zhang, Yiye [1 ,2 ]
Fang, Yuxin [1 ,2 ]
Xiao, Shaobo [1 ,2 ]
Fang, Puxian [1 ,2 ]
Fang, Liurong [1 ,2 ]
机构
[1] Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan, Peoples R China
[2] Cooperat Innovat Ctr Sustainable Pig Prod, Key Lab Prevent Vet Med Hubei Prov, Wuhan, Peoples R China
来源
FRONTIERS IN IMMUNOLOGY | 2023年 / 14卷
关键词
porcine epidemic diarrhea virus (PEDV); trypsin-independent; chimeric virus; immunogenicity; neutralizing antibodies; CORONAVIRUS SPIKE PROTEIN; CELL ENTRY; NEUTRALIZING ANTIBODIES; FUSION PROTEIN; UNITED-STATES; PEDV; ACTIVATION; SWINE; IDENTIFICATION; MECHANISMS;
D O I
10.3389/fimmu.2023.1165606
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Porcine epidemic diarrhea virus (PEDV) is a re-emerging enteropathogenic coronavirus that causes high mortality in neonatal piglets. The addition of trypsin plays a crucial role in the propagation of PEDV, but also increases the complexity of vaccine production and increases its cost. Previous studies have suggested that the S2 ' site and Y976/977 of the PEDV spike (S) protein might be the determinants of PEDV trypsin independence. In this study, to achieve a recombinant trypsin-independent PEDV strain, we used trypsin-dependent genotype 2 (G2) PEDV variant AJ1102 to generate three recombinant PEDVs with mutations in S (S2 ' site R894G and/or Y976H). The three recombinant PEDVs were still trypsin dependent, suggesting that the S2 ' site R894 and Y976 of AJ1102 S are not key sites for PEDV trypsin dependence. Therefore, we used AJ1102 and the classical trypsin-independent genotype 1 (G1) PEDV strain JS2008 to generate a recombinant PEDV carrying a chimeric S protein, and successfully obtained trypsin-independent PEDV strain rAJ1102-S2 '(JS2008), in which the S2 (amino acids 894-1386) domain was replaced with the corresponding JS2008 sequence. Importantly, immunization with rAJ1102-S2 '(JS2008) induced neutralizing antibodies against both AJ1102 and JS2008. Collectively, these results suggest that rAJ1102-S2 '(JS2008) is a novel vaccine candidate with significant advantages, including no trypsin requirement for viral propagation to high titers and the potential provision of protection for pigs against G1 and G2 PEDV infections.
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页数:11
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