Application of organoselenium in inhibiting Candida albicans biofilm adhesion on 3D printed denture base material

被引:1
作者
Alawadi, Ahmad [1 ]
AbdulAzees, Parveez Ahmed [1 ]
Lin, Chun-Yen [1 ,2 ,3 ]
Haney, Stephan. J. J. [1 ]
Hanlon, John. P. P. [1 ]
Angelara, Konstantina [1 ]
Taft, Robert. M. M. [1 ]
Amaechi, Bennett. T. T. [1 ,4 ]
机构
[1] Univ Texas Hlth Sci Ctr San Antonio, Sch Dent, Dept Comprehens Dent, San Antonio, TX USA
[2] Triserv Gen Hosp, Sch Dent, Dept Family Dent & Oral Diag, Taipei, Taiwan
[3] Natl Def Med Ctr, Taipei, Taiwan
[4] Univ Texas Hlth Sci Ctr San Antonio, Sch Dent, Dept Comprehens Dent, 7703 Floyd Curl Dr, San Antonio, TX 78229 USA
来源
JOURNAL OF PROSTHODONTICS-IMPLANT ESTHETIC AND RECONSTRUCTIVE DENTISTRY | 2024年 / 33卷 / 05期
关键词
3D-printed denture; biofilm inhibition; Candida albicans; denture stomatitis; organoselenium; ORGANO-SELENIUM; CONTACT-LENSES; IN-VIVO; GENERATION; COLONIZATION; STOMATITIS;
D O I
10.1111/jopr.13733
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
PurposeDenture Stomatitis, a chronic mucosal inflammation associated with Candida albicans, is common among denture wearers. Several health conditions have been linked to chronic Candida infections. The complex, multifactorial nature of denture stomatitis requires the continuous pursuit of effective long-term solutions. The present in vitro study investigated the effect of incorporating organoselenium into 3D-printed denture base resin on C. albicans adhesion and biofilm formation. Materials and MethodsThirty disks were fabricated using 3D-printed denture base resin and assigned to three experimental groups (10/group): disks without organoselenium (control), disks with 0.5% organoselenium (0.5%SE), and disks with 1% organoselenium (1%SE). Each disk was incubated with approximately 1 x 10(6) cells/mL of C. albicans for 48 h. Microbial viability (CFU/mL) was quantified by the spread plate method, while Confocal laser scanning microscopy and scanning electron microscope were performed for quantifying the biofilm thickness and examining biofilm morphology, respectively. Data were analyzed using One-way ANOVA with Tukey's multiple comparisons test. ResultsCFU/mL was significantly (p < 0.05) higher in Control when compared with 0.5%SE and 1%SE, but no significant difference between 0.5%SE and 1%SE. A similar trend was observed with biofilm thickness except that there was no significant difference between the Control and 0.5%SE. There was C. albicans biofilm adhesion on the Control disks, with yeast cells and hyphae formation, whereas on 0.5%SE and 1%SE, there was inhibition of yeast cells transition to hyphae formation. ConclusionsIncorporation of organoselenium into 3D-printed denture base resin was effective in reducing C. albicans biofilm formation and growth on denture base material.
引用
收藏
页码:460 / 466
页数:7
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