How Does Brazilian Green Propolis Act in Normal and Tumor Cells? Identification of Compounds in or out Monocytes or HEp-2 Cells

被引：1

作者：

Farlow, Anthony ^{[1
]}

Crevelin, Eduardo Jose ^{[2
]}

Santiago, Karina Basso ^{[1
]}

Cardoso, Eliza de Oliveira ^{[1
]}

da Silva, Livia Matsumoto ^{[1
]}

Bastos, Jairo Kenupp ^{[3
]}

Sforcin, Jose Mauricio ^{[1
]}

机构：

[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Chem & Biol Sci, Campus Botucatu, BR-18618691 Botucatu, SP, Brazil

[2] Univ Sao Paulo, Sch Philosophy Sci & Languages, Dept Chem, BR-14040901 Ribeirao Preto, SP, Brazil

[3] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Pharmaceut Sci, BR-14040903 Ribeirao Preto, SP, Brazil

来源：

CHEMISTRY & BIODIVERSITY | 2023年 / 20卷 / 05期

关键词：

chemical constituents; GC; MS; HEp-2; cells; monocytes; propolis; CHEMICAL-COMPOSITION; BIOLOGICAL-PROPERTIES; CYTOKINE PRODUCTION; CAFFEIC ACID; IN-VITRO; EXPRESSION;

D O I：

10.1002/cbdv.202300348

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to identify propolis compounds after incubation of normal and tumor cells (monocytes and HEp-2 cells, respectively) with Brazilian green propolis, in the lysate and supernatant of cell cultures and within these cells by gas chromatography-mass spectrometry (GC/MS). Cinnamic acid derivatives were generally localized in the lysate of both cell lines after incubation, suggesting these compounds are actively transported across the membrane into the cytoplasm. Terpenes were also found in the lysate. Artepillin C, in contrast, was localised only in the supernatant. Some constituents were unobservable after incubation, especially in monocytes, suggesting the compounds had been degraded. Our findings shed light on the possible sites of action (intracellular or via a cell membrane protein) and the bioavailability of various constituents of propolis, as well as possible modes of delivery of bioactive constituents.

引用

页数：7