Analysis of Pseudomonas aeruginosa Isolates from Patients with Cystic Fibrosis Revealed Novel Groups of Filamentous Bacteriophages

被引:3
作者
Evseev, Peter [1 ,2 ]
Bocharova, Julia [1 ]
Shagin, Dmitriy [1 ]
Chebotar, Igor [1 ]
机构
[1] Pirogov Russian Natl Res Med Univ, Lab Mol Microbiol, Ostrovityanova 1, Moscow 117997, Russia
[2] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Lab Mol Bioengn, Miklukho Maklaya 16-10, Moscow 117997, Russia
来源
VIRUSES-BASEL | 2023年 / 15卷 / 11期
关键词
filamentous bacteriophages; Pf; Pseudomonas phages; Pseudomonas aeruginosa; bacteriophage taxonomy; VIBRIO-CHOLERAE; PHAGE; GENOMES;
D O I
10.3390/v15112215
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Pseudomonas aeruginosa is an opportunistic pathogen that can cause infections in humans, especially in hospital patients with compromised host defence mechanisms, including patients with cystic fibrosis. Filamentous bacteriophages represent a group of single-stranded DNA viruses infecting different bacteria, including P. aeruginosa and other human and animal pathogens; many of them can replicate when integrated into the bacterial chromosome. Filamentous bacteriophages can contribute to the virulence of P. aeruginosa and influence the course of the disease. There are just a few isolated and officially classified filamentous bacteriophages infecting P. aeruginosa, but genomic studies indicated the frequent occurrence of integrated prophages in many P. aeruginosa genomes. An analysis of sequenced genomes of P. aeruginosa isolated from upper respiratory tract (throat and nasal swabs) and sputum specimens collected from Russian patients with cystic fibrosis indicated a higher diversity of filamentous bacteriophages than first thought. A detailed analysis of predicted bacterial proteins revealed prophage regions representing the filamentous phages known to be quite distantly related to known phages. Genomic comparisons and phylogenetic studies enabled the proposal of several new taxonomic groups of filamentous bacteriophages.
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页数:17
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