Human GBP1 Is Involved in the Repair of Damaged Phagosomes/Endolysosomes

被引：8

作者：

Buijze, Hellen ^{[1
]}

Brinkmann, Volker ^{[2
]}

Hurwitz, Robert ^{[3
]}

Dorhoi, Anca ^{[4
,5
]}

Kaufmann, Stefan H. E. ^{[1
,6
,7
]}

Pei, Gang ^{[4
]}

机构：

[1] Max Planck Inst Infect Biol, Dept Immunol, Charite Pl 1, D-10117 Berlin, Germany

[2] Max Planck Inst Infect Biol, Microscopy Core Facil, Charite Pl 1, D-10117 Berlin, Germany

[3] Max Planck Inst Infect Biol, Prot Purificat Facil, Charite Pl 1, D-10117 Berlin, Germany

[4] Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, Inst Immunol, D-17493 Greifswald, Germany

[5] Univ Greifswald, Fac Math & Nat Sci, D-17489 Greifswald, Germany

[6] Max Planck Inst Multidisciplinary Sci, Emeritus Grp Syst Immunol, Fassberg 11, D-37077 Gottingen, Germany

[7] Texas A&M Univ, Hagler Inst Adv Study, College Stn, TX 77843 USA

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2023年 / 24卷 / 11期

关键词：

guanylate-binding proteins; endolysosomal damage; Mycobacterium tuberculosis; Listeria monocytogenes; GUANYLATE-BINDING-PROTEINS; PATHOGEN-CONTAINING VACUOLES; LEUCINE METHYL-ESTER; HOST-DEFENSE; PHOSPHATIDYLINOSITOL; 4-KINASES; INFLAMMATORY CYTOKINES; NUCLEOTIDE-BINDING; AIM2; INFLAMMASOME; ACTIVATION; GALECTIN-3;

D O I：

10.3390/ijms24119701

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mouse guanylate-binding proteins (mGBPs) are recruited to various invasive pathogens, thereby conferring cell-autonomous immunity against these pathogens. However, whether and how human GBPs (hGBPs) target M. tuberculosis (Mtb) and L. monocytogenes (Lm) remains unclear. Here, we describe hGBPs association with intracellular Mtb and Lm, which was dependent on the ability of bacteria to induce disruption of phagosomal membranes. hGBP1 formed puncta structures which were recruited to ruptured endolysosomes. Furthermore, both GTP-binding and isoprenylation of hGBP1 were required for its puncta formation. hGBP1 was required for the recovery of endolysosomal integrity. In vitro lipid-binding assays demonstrated direct binding of hGBP1 to PI4P. Upon endolysosomal damage, hGBP1 was targeted to PI4P and PI(3,4)P2-positive endolysosomes in cells. Finally, live-cell imaging demonstrated that hGBP1 was recruited to damaged endolysosomes, and consequently mediated endolysosomal repair. In summary, we uncover a novel interferon-inducible mechanism in which hGBP1 contributes to the repair of damaged phagosomes/endolysosomes.

引用

页数：19

共 73 条

[1] An interferon-inducible neutrophil-driven blood transcriptional signature in human tuberculosis [J].

Berry, Matthew P. R. ;

Graham, Christine M. ;

McNab, Finlay W. ;

Xu, Zhaohui ;

Bloch, Susannah A. A. ;

Oni, Tolu ;

Wilkinson, Katalin A. ;

Banchereau, Romain ;

Skinner, Jason ;

Wilkinson, Robert J. ;

Quinn, Charles ;

Blankenship, Derek ;

Dhawan, Ranju ;

Cush, John J. ;

Mejias, Asuncion ;

Ramilo, Octavio ;

Kon, Onn M. ;

Pascual, Virginia ;

Banchereau, Jacques ;

Chaussabel, Damien ;

O'Garra, Anne .

NATURE, 2010, 466 (7309) :973-U98

[2] Transcriptional Blood Signatures Distinguish Pulmonary Tuberculosis, Pulmonary Sarcoidosis, Pneumonias and Lung Cancers [J].

Bloom, Chloe I. ;

Graham, Christine M. ;

Berry, Matthew P. R. ;

Rozakeas, Fotini ;

Redford, Paul S. ;

Wang, Yuanyuan ;

Xu, Zhaohui ;

Wilkinson, Katalin A. ;

Wilkinson, Robert J. ;

Kendrick, Yvonne ;

Devouassoux, Gilles ;

Ferry, Tristan ;

Miyara, Makoto ;

Bouvry, Diane ;

Dominique, Valeyre ;

Gorochov, Guy ;

Blankenship, Derek ;

Saadatian, Mitra ;

Vanhems, Phillip ;

Beynon, Huw ;

Vancheeswaran, Rama ;

Wickremasinghe, Melissa ;

Chaussabel, Damien ;

Banchereau, Jacques ;

Pascual, Virginia ;

Ho, Ling-pei ;

Lipman, Marc ;

O'Garra, Anne .

PLOS ONE, 2013, 8 (08)

[3] Intracellular Trafficking of Guanylate-Binding Proteins Is Regulated by Heterodimerization in a Hierarchical Manner [J].

Britzen-Laurent, Nathalie ;

Bauer, Michael ;

Berton, Valeria ;

Fischer, Nicole ;

Syguda, Adrian ;

Reipschlaeger, Simone ;

Naschberger, Elisabeth ;

Herrmann, Christian ;

Stuerzl, Michael .

PLOS ONE, 2010, 5 (12)

[4] TRIMs and Galectins Globally Cooperate and TRIM16 and Galectin-3 Co-direct Autophagy in Endomembrane Damage Homeostasis [J].

Chauhan, Santosh ;

Kumar, Suresh ;

Jain, Ashish ;

Ponpuak, Marisa ;

Mudd, Michal H. ;

Kimura, Tomonori ;

Choi, Seong Won ;

Peters, Ryan ;

Mandell, Michael ;

Bruun, Jack-Ansgar ;

Johansen, Terje ;

Deretic, Vojo .

DEVELOPMENTAL CELL, 2016, 39 (01) :13-27

[5]

CHENG YSE, 1983, J BIOL CHEM, V258, P7746

[6] Mammalian phosphatidylinositol 4-kinases as modulators of membrane trafficking and lipid signaling networks [J].

Clayton, Emma L. ;

Minogue, Shane ;

Waugh, Mark G. .

PROGRESS IN LIPID RESEARCH, 2013, 52 (03) :294-304

[7] Brucella abortus Triggers a cGAS-Independent STING Pathway To Induce Host Protection That Involves Guanylate-Binding Proteins and Inflammasome Activation [J].

Costa Franco, Miriam M. ;

Marim, Fernanda ;

Guimaraes, Erika S. ;

Assis, Natan R. G. ;

Cerqueira, Daiane M. ;

Alves-Silva, Juliana ;

Harms, Jerome ;

Splitter, Gary ;

Smith, Judith ;

Kanneganti, Thirumala-Devi ;

de Queiroz, Nina M. G. P. ;

Gutman, Delia ;

Barber, Glen N. ;

Oliveira, Sergio C. .

JOURNAL OF IMMUNOLOGY, 2018, 200 (02) :607-622

[8] PIP2 and PIP3: Complex roles at the cell surface [J].

Czech, MP .

CELL, 2000, 100 (06) :603-606

[9] Extensive characterization of IFN-induced GTPases mGBP1 to mGBP10 involved in host defense [J].

Degrandi, Daniel ;

Konermann, Carolin ;

Beuter-Gunia, Cornelia ;

Kresse, Alexandra ;

Wuerthner, Jan ;

Kurig, Stefanie ;

Beer, Sandra ;

Pfeffer, Klaus .

JOURNAL OF IMMUNOLOGY, 2007, 179 (11) :7729-7740

[10] Murine Guanylate Binding Protein 2 (mGBP2) controls Toxoplasma gondii replication [J].

Degrandi, Daniel ;

Kravets, Elisabeth ;

Konermann, Carolin ;

Beuter-Gunia, Cornelia ;

Kluempers, Verena ;

Lahme, Sarah ;

Wischmann, Eva ;

Mausberg, Anne K. ;

Beer-Hammer, Sandra ;

Pfeffer, Klaus .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (01) :294-299

← 1 2 3 4 5 6 7 8 →