Single-molecule localization microscopy goes quantitative

被引：8

作者：

Scalisi, Silvia ^{[1
,2
]}

Pisignano, Dario ^{[3
]}

Zanacchi, Francesca Cella ^{[2
,4
]}

机构：

[1] Univ Trento, Dept Cellular Computat & Integrat Biol CIBIO, Trento, Italy

[2] Ist Italiano Tecnol, Nanoscopy & NICIIT, Genoa, Italy

[3] Univ Pisa, Dipartimento Fis E Fermi, Pisa, Italy

[4] Univ Pisa, Dipartimento Fis E Fermi, Largo B Pontecorvo 3, Pisa, Italy

来源：

MICROSCOPY RESEARCH AND TECHNIQUE | 2023年 / 86卷 / 04期

关键词：

fluorescence microscopy; quantitative stochastic optical reconstruction microscopy (STORM); single-molecule localization; super-resolution microscopy; OPTICAL RECONSTRUCTION MICROSCOPY; SUPERRESOLUTION MICROSCOPY; PROTEIN HETEROGENEITY; STOICHIOMETRY; PROBES; TRACKING; PALM; STANDARDS; ADHESION; EMITTERS;

D O I：

10.1002/jemt.24281

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

In the last few years, single-molecule localization (SMLM) techniques have been used to address biological questions in different research fields. More recently, super-resolution has also been proposed as a quantitative tool for quantifying protein copy numbers at the nanoscale level. In this scenario, quantitative approaches, mainly based on stepwise photobleaching and quantitative SMLM assisted by calibration standards, offer an exquisite tool for investigating protein complexes. This primer focuses on the basic concepts behind quantitative super-resolution microscopy, also providing strategies to overcome the technical hurdles that could limit their application.

引用

页码：494 / 504

页数：11

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