Fluorescent glycopolymers for probing plant glycan-binding proteins

被引：0

作者：

Tuzikov, Alexander B. ^{[1
]}

Ovchinnikova, Tatiana V. ^{[1
]}

Nizovtsev, Alexey V. ^{[1
]}

Bovin, Nicolai V. ^{[1
]}

Gorshkova, Tatiana A. ^{[2
]}

Chernova, Tatiana E. ^{[2
]}

Shilova, Nadezhda V. ^{[1
]}

机构：

[1] Russian Acad Sci, M M Shemyakin Yu A Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

[2] Russian Acad Sci, Kazan Sci Ctr, Kazan Inst Biochem & Biophys, Kazan 420111, Russia

来源：

MENDELEEV COMMUNICATIONS | 2024年 / 34卷 / 01期

基金：

俄罗斯科学基金会;

关键词：

fluorescent probes; glycan-binding proteins; glycopolymers; plant histochemistry; Cy3; Cy5;

D O I：

10.1016/j.mencom.2024.01.004

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The synthesis of glycoprobes based on water-soluble polyacrylamide with a custom content of any low molecular weight fluorescent label is described. The synthesis is carried out in a stepwise manner: in the first step, the polymer is strictly dose-modified with a side substituent-linker containing an amino group; in the second step, the amino group is quantitatively acylated with an activated ester of the dye. The probes are designed to identify and study carbohydrate-binding proteins of plant cells.

引用

页码：13 / 14

页数：2

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